Analysis Of The Second Structure Of The Influenza A And Implementation Of Web Server Of Multi-scale RNA Comparison Based On RNA Triple Vector Curve Representation

Influenza is an acute infectious disease of the most widely distributed zoonotic in the earth, is the number one disease-causing virus of human disease, influenza virus has a long history, regularly outbreak. So far influenza virus more than2,000years of history, about every10to20years, mankind is attacked by a global influenza killer; influenza virus variability and sudden, often make human lose resistance to the sudden outbreak of a variant of influenza virus. In addition, the outbreak of the influenza virus are usually large-scale, often leads to tens of thousands or even millions of deaths and huge economic losses, and causing serious social panic. Currently, the infection pathogenic mechanism of influenza virus is still unclear, there are still too many unsolved mysteries. The subtype of influenza A is known as the "avian flu".The influenza A virus consists of eight negative single-stranded RNA fragments. Although we already know the hemagglutinin and neuraminidase genes play a very important role in the host adaptability, but the polymerase gene (the encoded polymerases fragment PA, PB1, PB2) and the nucleoprotein gene also play an important role in effective dissemination in host body and adapt to the new host Influenza as an RNA virus, the RNA structure is crucial in the viral life cycle of the virus appears, propagation, evolution, pathogencity, host specificity. With in-depth understanding of RNA, functional diversity and importance of the structure of RNA more and more cause for concern, analysis of the high-level structure of the viral RNA can help people better understand the evolution and pathogenesis of influenza.In this thesis, we use the minimum free energy algorithm for the calculation of the influenza virus folding free energy and the corresponding secondary structure; And analysis of the characteristics of the influenza A virus folding free energy, research in different years, different parts of the folding free energy distribution, the different subtypes of the free energy distribution in different years. Also analyzed the common structure of the different subtypes of the influenza A virus, and analyzed of the differences between the different subtypes of structure.Another work of the paper is to achieve the web server of a new RNA secondary structure comparison algorithm. The method is based on a new secondary structure representation of curves, triple vector curve representation, Based on this representation method, we use the multi-resolution characteristics of wavelet to propose a new multi-scale RNA structure metrics.The main achievement of this web server is to detect the RNA structural mutation, the triple vector curve representation of RNA second structure, and the comparison of RNA structure. The web server will provide the convenience for the biologists.