Lovo Cells Inhibited By Lactobacillus Fermentation Broth And Possible Mechanism

Objective: To detect the change of the cells growth, morphologicalcharacter and expression of Caspase-3and Bcl-2in the lovo cell line SW620treated by different concentrations of lactobacillus fermentation broth.Methods: SW620cells were cultured in L-15culture medium containing10%fetal calf serum. Treat the cell lines with different concentrations of lactobacillusfermentation respectively when cells were in good stage and observe the character atdifferent time intervals. Put sterilized cover slips in the culture dish before cellsinoculated whenever cell shape observation or cytochemical staining analysis wereneeded. After treatment, coverslips were collected and fixed properly according to theexperiment at purposes. Effects of lactobacillus fermentation on the cell proliferationand survival were examined through MTT assay and Trypan blue, and Flow cytometry(FCM) were used to determine the cell death pattern. Immunocytochemistry (ICC) andWestern-blot were used to analyze the statuses of apoptosis related gene Bcl-2andCaspase-3.The influence of Lactobacillus fermentation in cell cycle was determinedwith FCM.Results:1. Lactobacillus ferment not only suppresses the growth of lovo cells in adose and time related fashion but also induces cell differentiation and apoptosis: Cellsform has elliptic into spindle shape, nucleolus bigger, cell disruption and apoptosis.2.The lovo cells treated by lactobacillus ferment express the apoptosis protein is quitedifferent from before: The expression of Caspase-3and Bcl-2were respectively higherand lower in treated group than those in the control group.3.Cell cycle distribution ofSW620was greatly changed after treatment. That is proportion of G0/G1phase in thewhole cell cycle is increased, the other phase are decreased accordingly. Conclusion: This study confirms:1. Lactobacillus fermentation no significantside effects not only suppresses the growth of lovo cells in a dose and time relatedfashion but also induces the cells apoptosis. The mechanisms of lactobacillus fermentinhibiting the proliferation of SW620cells were possibly interrupting S phase.2. Theother mechanism of inhibition is by up-regulation of Caspase-3and down-regulation ofBcl-2by Lactobacillus fermentation...