Effect Of Yishen Capsule On JAK/STAT Signaling Pathway And α-SMA,FN Expression In Rats With Diabetic Nephropathy

Objective:To observe the effect and mechanism of Yishen capsule on urinary protein/24h, blood urea nitrogen (BUN), serum creatinine (Scr) and JAK/STAT, a-smooth muscle actin (a-SMA), fibronectin (FN) in rats with diabetic nephropathy.Methods:55SD rats, Random samples from10SD rats male as the normal control group (N). The successful rat models were selected and randomly divided into four groups:diabetic nephropathic model group, yishen capsule group, Losartan Potassium Tablets group and yishen capsule combined with Losartan Potassium Tablets group,10rats each group. The DN models were induced by one intraperitoneal injection of streptozotocin(STZ). All the rats received daily gavages for12weeks. The blood glucose(Glu), urinary protein/24h, BUN and Scr were measured after12weeks in each group respectively. The renal tissue pathological changes were observed under light microscope. The expression of the p-JAK2, p-STAT3, a-SMA and FN was measured by immunohistochemistry on kidney tissue.Results:After12weeks, the blood sugar of DN group were obviously higher than N group(P<0.05), while there was no clear difference between the three treatment groups (P<0.05).The24h urinary protein,Scr and BUN of DN group were higher than those in N group(.P<0.05), DN+Y group were lower than those in DN group(P<0.05), while DN+Y group were higher than DN+Y+L group(P<0.05), there were significant difference between DN+Y and DN+L group. Pathological results microscopically:HE staining showed that, in DN group than in the control group, glomerular hypertrophy, mesangial cell hyperplasia, mesangial widened obviously, inflammatory cell infiltration can be seen, renal interstitial edema, renal tubular injury, visible epithelial cells edema, broken, and Yishen Capsule in the treatment group changed significantly reduce. PAS staining, visible DN in model group increased glomerular mesangial cells, endothelial cells and renal tubular epithelial cell vacuolar glycogen, all these were decreased in DN+Y group. P-JAK2, p-STAT3, a-SMA, FN in the renal tissue of the rats in group N little or no expression, increased significantly compared with the normal control group, expression in renal tissue of rats in the DN group (P<0.05), Expression of DN+Y group and DN+L group than in DN group decreased significantly (P<0.05), No statistically significant difference between the two treatment groups (P<0.05), Group DN+Y+L compared with group DN+Y, p-JAK2, p-STAT3, α-SMA, FN expression was significantly reduced in renal tissue.Conclusion:Yishen Capsule could reduce24h protein urinary, decrease the expression of p-JAK2and p-STAT3of the DN rats nephridial tissue. Spearman rank correlation analysis showed a correlation between α-SMA、FN and p-JAK2、p-STAT3. These all suggest that Yishen Capsule could be improbably affect the JAK/STAT pathway to paly its protective effect of kidney.