Inhibitory Effect Of AEoTGE On In Vitro Cultured Human Keloid Fibroblasts

Objective: To study the inhibitory effect of AEoTGE on the proliferation andapoptosis of KFB in vitro and to survey the death rate.Methods: To utilize In vitro tissue explant culture to train the FB. samples ofhypertrophic scars were collected cultured, and only4-8passage cells were selected forexperiment; inverted microscope and transmission electron microscope were used toobserve the morphogenesis and ultrastructure of KFB; treat the KFB cells with containedherb culture medium of AEoTGE in different concentrations(3.125，6.25，12.5，25，50，100g/L)．The effect of the contained herb culture medium on the proliferation and theIC50of KFB was observed with MTT assay and Edμ Effect of the culture mediumontaining AEoTGE on apoptosis of KFB was detected by flow cytometry.Resμlts: It was shown that AEoTGE could inhibit the proliferating activity ofKFB and it held the characters of strengthened inhibitory effect depending uponparameters of the medicinal concentration within the range of3.125-100g/L at24h,andthe culture medium containing AEoTGE could increase the apoptosis rate of the KFBcompared with blank control group(p<0.05).the IC50of the culture mediumcontaining AEoTGE is35g/L.With the help of FITC-Annexin V/PI,itshowed that rate ofthe culture medium containing AEoTGE on apoptosis of KFB was72.1, blank controlgroup was23.5(p<0.05).Conclusion: AEoTGE could significantly inhibit the proliferating activity of KFBand can significantly induce apoptosis to KFB,its IC50was35g/L. rate of apoptosiswas72.1.