Cellular Mechanisms Underlying The Effects Of MrgC Receptors On CFA-evoked Inflammatory Pain

The Mas-related G protein-coupled receptors (Mrgs), which was found in2001, is a novel receptor superfamily. Study has been demonstrated that MrgC alleviates CFA-induced inflammatory pain. It implies significance in physiological and pharmaceutical sciences of pain processing. This study was conducted by using western blot techniques to investigate the mechanisms of the analgesia effect of intrathecal activation of MrgC regulating CFA-evoked inflammatory pain. We examined the effects of i.t.(intrathecal) administration of MrgC agonists, BAM8-22(30nmol/10μl,20min) after i.pl. CFA (200μl,24h). The results found that Gq/11protein levels of expression in membrane were significantly up-regulated during CFA-induced inflammation compared to controls and inhibite this effort after i.t administration of BAM8-22. But Gi protein and Gs protein levels were not changed in any groups. Further research also found that intrathecal administration of BAM8-22can inhibit the efforts of CFA-induced to increase expression of PKCy and ERK1/2. These results confirmed that activation of MrgC receptors can inhibit expression of Gq/11protein、PKCγ and ERK1/2and we can preliminarily expounds Cellular mechanisms underlying the effects of MrgC receptors on CFA-evoked inflammatory pain.