| Objective:The purpose of this paper is to study the level of LOX-1in aorta plaque of mouse models of atherosclerosis which ApoE is knocked out.The changes of LOX-1after they treated by probucol, rosuvastatin and two joint.Correlation analysis was carried out on the active plaque area and lumen diameter.Try to explore the role of LOX-1in the pathogenesis of atherosclerosis and the role of the influence on the level of LOX-1in anti atherosclerosis which brought by probucol, rosuvastatin and two joint.The inhibition of level of LOX-1may provide a new strategy for the treatment of atherosclerotic cardiovascular disease.Hope to bring the theoretical guidance to the diagnosis, treatment and prognosis of patients with atherosclerosis.Methods:1.Molding:Male Apo-E knockout mice40,8weeks of age. Using the method of random Numbers they are divided into four groups. Four groups are control group (n=10),pobucol group (n=10),rosuvastatin group(n=10) and combination group(n=10).Control group and rosuvastatin group fed with high fat diet.Probucol group and combination group received probucol feed.In addition the rosuvastatin group and combined group received rosuvastatin gavage once a day.They are made the models of atherosclerosis after fed continuously12weeks in the SPF animal laboratory.2.Materials:The control mice which fed12weeks are dissected under a operation microscope after anesthesia.The number of plaques,location and size can be clearly observed under the microscope after the aorta is ully exposed.Preliminary sure building success through it.Then taking pictures one by one by digital camera through the microscope lens. We separated the brachiocephalic trunk, thoracic aorta, abdominal aorta,and iliac artery bluntiy from aortic root and then cut them from the aortic root and iliac artery and sacrificed them.3.Pathological observation:The upper part of the aorta is cut into sections,embedding aorta parallelly and vertically by regular alcohol, dehydration paraffin.The cross-sectional is made5um thick serial section. Patching the obvious place for HE staining and LOX-1immunofluorescence staining. Observing the vascular wall thickness, luminal diameter, intimal hyperplasia and plaque under optical microscope.To observe the expression of target protein LOX-1and plaques of specimens in the confocal microscope.Results:1.The weight of animals were significantly increased at the end of the experiment than before the experiment(P<0.05). But on the same subject to the intake, drug-intervention group weight is significantly less than the control group. This difference was statistically significant(P<0.05).2.The observation under the surgery microscope, visible:the blood vessels of against group is ash white and obviously stiff..There are a lot of obvious plaque in the root of aorta, brachiocephalic trunk, thoracic aorta and abdominal aorta.The part of the aorta of probucol group is pale and plaques of thoracic aortic is more also.The aorta of rosuvastatin group is pink and scattered patches can be seen.The aorta of combined group is pink and the wall of blood vessel is soft and the plaque is rare.3. The lumen area has no statistically significant difference in each experimental group(P<0.05). The difference of plaque area and relative patch area in experimental have statistics significance(P<0.05).Comparison among groups:There was no significant difference in the luminal area between groups(P>0.05).The plaque area and relative area is consistent.The treatment groups are less than the control group significantly(P<0.05). The combined group is less than any single medication significantl(p=0.042).The rosuvastatin group is less than the probucol group significantly(P<0.05).4. LOX-1staining positive area is significantly greater than the probucol group(P<0.05). There is no significant positive expression in rosuvastatin group and joint group(P>0.05).This result and HE staining in front of patch size is parallel.Conclusion: 1.High fat feeding animal models of atherosclerosis in apoE-/-is ideal for small mouse.Control group were seen in different parts of the aorta significantly AS plaque.2.Probucol, united drug rosuvastatin alone and combined were higher than those in the control group significantly reducing aortic plaque area and the expression of LOX-1(P<0.05).3. The aortic plaque formation in mice and the decrease of LOX-1on the expression of rosuvastatin group and combined group are superior than probucol(P<0.05).4.The combined group is simply superior in reducing arterial plaque area rosuvastatin group(P<0.05), but in the inhibition of LOX-1on the expression, there is no difference(P>0.05).5.Generally speaking, the plaque area and the trend of the expression of LOX-1having parallel, i.e. the amount of expression of LOX-1is proportional to the area of plaque. It is speculated that LOX-1process plays an important role in plaque formation, and the two drugs can act to slow down the process of the AS in the LOX-1target. |
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