Experimental Study On Ultrasound Transdermal Drug Delivery Of Saffron Cream In Vitro

Saffron is the clinical treatment of subacute and chronic traumatic injury more commonly used drugs for blood circulation, and transdermal drug delivery system can avoid hepatic first-pass effect and gastrointestinal failure. Due to the influence of the physicochemical properties of the drug and the stratum corneum of the skin and other factors, the drug permeability is very small. This study uses ultrasound physical therapy and chemical enhancers method, research of ultrasonic on transdermal absorption effect, suitable for this kind of water soluble each ultrasonic physical parameters of drug permeation, and provide experimental basis for the reasonable application of selected high-end ultrasonic therapy instrument, ultrasonic dose of ultrasonic compound Chinese medicine into preparation of single herb the selection of.Research methods:In this study, the use of ultrasonic transdermal experiment in vitro on the abdominal skin of male rats in transdermal experiment, ultrasonic intensity of0.1W/cm2, different ultrasonic frequencies (300-1400KHz), and different time (3,5,10,15,20,30min) fixed contact condition of compound safflower, to observation of cumulative permeation quantity of traditional Chinese medicine respectively with and without chemical permeation. High performance liquid chromatography was used to determine the content of hydroxysafflor yellow A, using statistical methods for data processing.The research results:1. Ultrasound in vitro transdermal experiment, ultrasonic frequency was1000and900KHz of hydroxysafflor yellow A permeation amount is relatively large, and with a significant difference between the300,400,500,600,700,800,1100,1200,1300,1400KHz (P<0.01).2. There was no significant difference between the effects of time between the3min,5min,10min (P>0.05), but significant difference with15, 20min (P<0.01), the difference between15min and20min was not significant (P>0.05).3. Red cream added2%azone and10%propylene glycol had significant difference with not penetration enhancers on the cumulative permeation amount (P<0.01).Conclusion:1. In the ultrasonic stationary contact method, intensity of0.1W/cm2, ultrasonic frequency in300-1400KHz of hydroxysafflor yellow A can play a promoting effect, cumulative permeation quantity of ultrasonic frequency of hydroxysafflor yellow A penetration effect from large to small is divided into three levels, the first level are1000KHz,900KHz; second level are300KHz,800KHz,1100KHz,1200KHz; third level are400KHz,500KHz,600KHz,700KHz,1300KHz,1400KHz.2. In ultrasonic transdermal permeation of hydroxysafflor yellow A medicine experiment content increases with the ultrasonic action time. In the ultrasonic intensity of0.1W/cm2, the hydroxysafflor yellow A transdermal combination conditions for best result is frequency of1000KHz, reaction20min, the addition of chemical enhancers2%azone and10%propylene glycol.3. In the low intensity ultrasound0.1W/cm2transdermal experiment, adding chemical enhancers can more increase the cumulative permeation quantity of hydroxysafflor yellow A, in order to achieve a better therapeutic effect, adding a certain proportion of chemical enhancers will be better in the preparation of a compound traditional Chinese medicine ointment safflower when ultrasonic.4. In the use of low intensity ultrasound fixed contact penetration and safflower for treating sports injuries, injury shallow site can choose1000KHz or900KHz; damage deeper parts of frequency selective300KHz. In the acute stage, sports injury effects of time small area within the control of lOmin; in the sports injury in subacute and chronic stage injuries, larger area can be extended to10-20min.