Subgingival Flora Detection Of Chronic Periodontitis Patients With Type2Diabetes And The Relationship Between Blood Fat And Blood Sugar

Objective:To analyze and detect the existence and prevalence of eight periodontal pathogens in the subgingival plaque of the Type2diabetes mellitus (T2DM) patients with chonic periodontitis (CP), and evaluate the relationship between the subgingival flora and the Fasting plasma glucose(FPG), Glycated hemoglobin Alc(HBAlc), Triglyceride(TG), Low density lipoprotein cholesterol(LDL-C), High density lipoprotein cholesterol(HDL-C) of patient with T2DM, and to discuss the periodontal pathogens’infection influence on the patients with T2DM.Methods:30subgingiva plaque samples of CP patients,48subgingival plaque samples of T2DM patients with CP were collected, and their bacteria DNA was extracted. The ordinary Polymerase chain reaction (PCR) was employed to detecte the presence of8periodontal pathogens and Real-time fluorescence quantitative (FQ-PCR) was employed to detecte the quantity of8periodontal pathogens,mcluding Actinobacillus actinomycetemcomitans(Aa), Porphyromonas gingivalis(Pg), Treponema denticola(Td), Prevotella intermedia(Pi), Tannerella forsythus(Tf), Fusobacterium nucleatum(Fn), Campylobacter rectus(Cr), Peptostreptococcus micros(Pm), and Sperman correlation was used to analyze the relationship between the periodontal pathogens and FPG, HBA1c, TG, LDL-C and HDL-C of T2DM patients with CP.Results:48T2DM patients with CP, and30CP patients, their detection rates of eight periodontal pathogens are compared, the results show no statistical significance(P>0.05). In the subgingival plaque of CP+T2DM group Pg, Pi, Pm, Cr, Aa, Fn detected100%, Td and Tf both detected97.9%; In the subgingival plaque of CP group subgingival plaque samples:Pg100%、Pi100%、Pm100%. Cr100%. Aa100%. Fn90%. Td73.3%. Tf96.7%. The proportion of8periodontal pathogens in subgingival plaque of two groups shows that there are statistical significance with Fn, Td, Pi(P<0.05). The proportion of Fn was negatively correlated with the value of HBA1c.The proportion of Pg was negatively correlated with the level of HDL-C and positively correlated with the value of TGConclusions:The distribution of8periodontal pathogens in subgingival plaque of T2DM patients with CP is similar to the CP patients, but the proportion of Td, Fn, Pi is significantly higher with CP+T2DM patients. From which we may infer that CP and T2DM have the same mechanisms as CP patients on the occurrence of periodontal inflammatory, meanwhile, T2DM patients with CP have greatly influenced on the colonization of Td, Fn, Pi. Poorly controlled state of blood sugar may affect on the adherency of Fn, meanwhile, there is closely related to Pg and blood lipid status, above all have suggested that the State of T2DM patients with high blood sugar and high blood fat may has some impact on the colonization of periodontal pathogens.