| Obstract To investigate the expression of microRNA-146a(miR-146a),TNFreceptor-associated factor6(TRAF6) gene and IL-1receptor-associated kinase1(IRAK1) genen in the peripheral mononuclear cells (PBMCs)，and IL-6, TNF-α, IL-1βin serum of patients with ankylosing spondylitis(AS) and as well as their relationshipwith the disease activity. Explore miR-146a,TRAF6,IRAK1in AS pathogenesis.Methods The study was performed in45patients with AS from the Department ofRheumatology and Immunology，Anhui Medical University Affiliated ProvincialHospital, which includeed36males and9females, and met the AS New Yorkrevised classification criteria in1984.20healthy controls. Infected patients with ahistory nearly two weeks was excluded. Their average age was29.84±7.47yrs. Activitygroups and stable group was divided depending on whether the joint pain and BASDAI≥4. The untreated group was untreated or withdrawal of more than one month, and thetreatment group was treated by using NSAIDs and DMARDs drugs for more than threemonths.include17males3females,mean age27±2yrs. ex-and age-matched, served asa control group. The control group was normal healthy volunteers,which includeed16males and6females and their average age was26.32±1.55yrs. There was no significantdifference in age and gender between AS patients group and control group. Expressionof miR-146a,TRAF-6and IRAK-1in peripheral blood mononuclear cells was studiedusing real-time polymerase chain reaction (qRT-PCR), and the expression levels of IL-6,TNF-α, IL-1β was detected using Enzyme-linked immunosorbent assay in45AS patients and22healthy controls. The indicators of disease activity were Bath ankylosingspondylitis disease activity index (BASDAI), Erythrocyte sedimentation rate (ESR),C-reactive protein (CRP), Immunoglobulin (Ig), Duration of morning stiffness. Therelationship was analysed in AS patients between the relative expression levelsmiR-146a,TRAF6,IRAK1and BASDAI,ESR,CRP,Ig concentration. Non-parametrictest, t test, One-way ANOVA, pearson and spearman correlation analysis were used forstatistical analysis.Results①The relative expression level of miR-146a observed in PBMCs of ASpatients was significantly higher than that in normal acontrol group [1.46(0.39,4.79)and0.81(0.17,1.90), P<0.05],that was significantly higher in active AS patients group thanthat in inactive patients [2.93(0.95,7.95) and0.54(0.28,1.69), P <0.05], there was notdiffer between the treated group and untreated group [1.28(0.31,2.37) and2.22(0.49,7.71), P>0.05].②There was significantly difference in the relative expression levelof IRAK-1between AS patients and the normal acontrol group. IRAK1wassignificantly higher in AS patients than that in normal acontrol group(1.44±0.72,1.13±0.43, P<0.05).but did not differ between active AS patients group and inactivepatients group as well as treated group and untreated group (1.50±0.85,1.37±0.54;1.55±0.74,1.33±0.70, P>0.05).③TRAF6expression was obviously lower in ASpatients than that in normal acontrol group (1.27±0.61,1.68±0.81, P<0.05), and thatwas also significantly lower in untreated group and active group than that in normalacontrol group (1.10±0.72,1.68±0.81;1.09±0.53,1.68±0.81, P<0.05).④The relativeexpression level of TNF-α was obviously higher in AS patients than that in normalacontrol group(43.99±24.4and30.74±23.19，P<0.05), that was significantly higher inactive AS patients group than that in inactive patients(61.93±19.97and25.25±10.49，P<0.05), there was not differ between the treated group and acontrol group(38.45±21.42and30.74±23.19，P>0.05).⑤The relative expression level of IL-1β was obviously higher in AS patients than that in normal acontrol group(43.75±19.81and32.01±21.05，P<0.05), that was significantly higher in active AS patients group than that in inactivepatients(57.56±16.47and29.32±10.53，P<0.05), there was not differ between thetreated group and acontrol group(37.95±18.37and32.01±21.05，P>0.05).⑥IL-6expression was not differ between AS group and acontrol group(33.69±14.48and28.30±14.41，P>0.05),but that was significantly higher in active AS patients group thanthat in inactive patients(43.72±11.16and23.20±9.12， P<0.05).⑦Significantlypostitive correlation was observed between the miR-146a level and BASDAI, Durationof morning stiffness (r=0.557，P=0.000; r=0.363，P=0.018), and Significantly postitivecorrelation was observed between the miR-146a level and TNF-α, IL-1β, IL-6(r=0.317，P=0.009；r=0.333，P=0.006；r=0.342，P=0.005), Expression level of IRAK1was foundto have significantly negative correlation with IgM (r=-0.313, P=0.046).Conclusion①m iR-146a expression is upregulated in patients with AS, and it may bea potentially useful marker of disease activity in AS patients;②The abnormalexpression of IRAK1,TRAF6in AS patients may play a role in the pathogenesis of AS. |
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