Expression And Clinical Significance Of HIF-1α And CAIX In Cervical Squamous Cell Carcinoma

Objective：To study the over expression and clinical significance of hypoxia-inducedfactor-1α（HIF-1α）and carbonic anhydraseⅨ (CAⅨ) in cervical squamous cellcarcinoma(SCC)；To investigate the role HIF-1α and CAⅨ playin the carcinogenesisand progression of cervical squamous carcinoma, attempt to provide the evidence ofmolecular pathology in the cervical squamous carcinoma.Methods：choose30cases of normal cervical squamous epithelium,55cases of cervicalintraepithelial neoplasia (CIN) and80cases of SCC, Cut blockes in the middle with4m thickness into three pieces standby, one was stained for HE, the other two werestained for HIF-1α and CAⅨIHC (EliVision plus), respectively.Results：The positive expression rates of HIF-1α were76.25%respectively in SCC，significantly higher than those in CIN43.64%and normal cervical squamousepithelium0.00%（P<0.01）. The positive expression rates of CAⅨ were66.25%，respectively in SCC，significantly higher than those in CIN40.00%and normal cervicalsquamous epithelium0.00%（P<0.05）.The positive expression rates of HIF-1α andCAⅨ in poor differentiated SCC were significantly higher than those in well ormoderated differentiated SCC（P <0.05）;HIF-1α and CAⅨ expression rates in the caseswith lymph node metastasis were significantly higher than those in without lymph nodemetastasis（P<0.05）; HIF-1α expression rates in the cases with clinical stageⅡweresignificantly higher than those in clinical stageⅠ.The expression of HIF-1α wassignificantly related to CAⅨ expression in SCC（P <0.05）.Conclusions：Over-expression of HIF-1α and CAⅨ plays an important role in thecarcinogenesis and progression of cervical squamous carcinoma. The overexpressionand high correlation of HIF-1αand CAⅨ in cervical squamous carcinoma support the3 feasibility of using targeted molecular therapy to treat SCC.