The Expression Of Twist In Gastric Carcinoma And Its Relationship With Epithelial Mesenchymal Transition

Objective:To investigate the differential expression of twist protein (Twist), signal transducer and activator of transcription3(Stat3), epithelial mesenchymal transition related factor (E-cad) in gastric cancer tissue and paracancerous, and analyze the correlation between the three proteins and the clinic pathological parameters of gastric cancer; to analyze the correlation of Twist, Stat3and E-cad; to investigate the significance in judging the expression of Twist, Stat3, E-cad in the prognosis of gastric cancer patients.Methods:Immunohistochemistry was used to detect the expressions of Twist, Stat3, E-cad in150cases of gastric carcinoma specimens and corresponding adjacent normal tissue. Analysis the relationship between Twist,Stat3,E-cad expression with gastric cancer patient’s age, gender, histological type of tumor, metastasis and TNM staging etc. Explore the possibility of using these two proteins as the prognosis of patients with gastric cancer indicator.Results:In gastric carcinoma Twist, Stat3, E-cad positive rate was57.3%,64.7%and 12%; in adjacent tissues, the positive rate was17.3%,10.8%and68%. Tumor tissues and adjacent tissues there were significant differences (P<0.05). Patients with Twist positive expression showed higher incidence of lymph node metastasis (P<0.05) and distant metastasis (P<0.05) than those with Twist negative expression. The expression of Stat3was significantly correlated TNM stages, metastasis and depth of invasion in gastric carcinoma (P<0.05).Conclusions:The expression of Twist and Stat3were closely related to the biological characteristics in gastric carcinoma; The Stat3activation may enhance the expression of Twist, E-cad; Detection of Twist, Stat3and E-cad may have certain value in predicting tumor prognosis invasion and metastasis. Objective:Observe the effect of inhibiting the expression of Twist in gastric cancer cell lines BGC-823through RNAi. And investigate the role of Twist in epithelial mesenchymal transition phenomenon, and its possible clinical application.Methods:According to Twist gene nucleotide sequence reported in Genbank and siRNA design principles, design and construct the expression vector psilencer3.1-Twist recombinant plasmid, then instantaneously transmit into human poorly differentiated gastric cancer cell lines BGC-823, using RT-PCR to detect the expression of Twist mRNA.Likewise RT-PCR was used to detect the expression of Twist, E-cad, N-cad and using Immunofluorescence to detect cell skeleton protein F-actin’s expression. The changes of cell invasion and metastasis ability were explored by Transwell chamber.Result:Recombinant plasmids were transiently transfected psilencer3.1-Twist, RT-PCR exhibit that Twist expression at the mRNA levels was significantly inhibited in BGC-823cell lines. RT-PCR also shows that Twist, N-cad’s expression were reduced after interfereing (P<0.05); expression of E-cadherin elatively higher (P<0.05). The location of F-actin has no alterntive in those groups. InTranswell chambers cells abilitiy of invasion and migration was significantly inhibited when slience the expression of Twist (P<0.05).Conclusions:Twist siRNA expression vector can effectively inhibit the Twist’s expression in mRNA and protein levels; Twist’s expression in BGC-823cell line was a key step in epithelial to mesenchymal transition in gastric cancer; Twist could improve the efficacy of gastric cancer and can be used as a potential target to extend their survival.