| Background Chronic heart failure is the end developed stage of a variety ofcardiovascular diseases in clinical. In recent years, the trend is upward and chronicheart failure poses a serious threat to human healthy. Patients with chronic heart failurein cardiac surgery and patients with coronary heart disease in non-cardiac surgery areoften associated myocardial ischemia/reperfusion injury. The prevention and controlmeasures need to be improved. Like morphine, fentanyl and other opioid analgesics,remifentanil preconditioning (RPC) on normal heart can produce myocardialprotection. However, it is not clear that RPC is effective on chronic heart failure. Thisarticle aims to investigate the effects of remifentanil preconditioning on AdriamycinHeart Failure following ischemia-reperfusion in isolated rat hearts.Methods All60adult male SD rats received2mg/kg adriamycin as an intravenous tailvein infusion once a week for6weeks at a total dose of12mg/kg to establish anadriamycin-induced chronic heart failure model. Heart failure model is successful forhigh-frequency ultrasound echocardiography confirmed EF (left ventricular ejectionfraction) and FS (fractional shortening) decreased by20-30%.60heart failure ratsrandomly divided into6groups (10each): control group (Sham), ischemia-reperfusiongroup (I/R), ischemia preconditioning group (IPC),10μg/L remifentanilprecondioning group (RPC1),30μg/L remifentanil precondioning group (RPC2),60μg/L remifentanil precondioning group (RPC3). All heart were linked to Langendorff apparatus. Left ventricular developed pressure (LVDP), maximal rate of left ventricularpressure of development(＋dp/dtmax), maximal rate of left ventricular pressure ofdecline (－dp/dtmax), heart rate (HR), coronary effluent (CF) were recorded and lactatedehydrogenase (LDH) were measured at the end of equilibration and5min,30min,90min of reperfusion. Myocardial infarct size were measured with TTC staining at theend of reperfusion. After reperfusion, phosphor-Akt and total Akt were determined byWestern blot analysis.Results No differences in baseline hemodynamics and LDH were observed among thegroups (P>0.05). Compared with group I/R, group RPC2and group RPC3resulted in asignificantly improved functional recovery, had a significant increase in LVDP,＋dp/dtmax,－dp/dtmax, CF and a significant decrease in IS(P<0.05). Meanwhile, groupRPC2and group RPC3also markedly increased the expression of p-Akt comparedwith group I/R.Conclusions Effects of ischemia preconditioning on adriamycin-induced heart failurefollowing ischemia-reperfusion in isolated rat hearts is disappeared. RPC mayeffectively protect the hearts against ischemia-reperfusion injure inadriamycin-induced chronic failure isolated rat hearts by activating PI3K/Akt signalpathway. |
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