Influences Of Benazepril And Irbesartan For Kallikrein1Expression On Renal In Diabetes Nephropathy Rats

Objective: To investigate the effect of blockage of renninangiote-nsin system for kallikrein1(KLK1)expression on renal indiabetes nephropathy rats.Methods:Sixty male SD rats were injected low dosestreptozotocin and fed with hish fat and sucrose diets to establishdiabetes nephropathy model.After success established the DN animalmodel,than the experimental rats were randomly divided into fivegroups, each group of ten rats,normal control group (groupA),diabetic nephropathy model group(group B),treat with benazeprilgroup(group C),treat with irbesartan group (group D),treat withbenazepril and irbesartan group (group E),Then all rats were killedafter8weeks.Kindney weight/body weight,24-hoursproteinuria,serum creatinine and urea nitrogen were measured toevaluate renal function.KLK1was stained by immunohistochemical(IHC)technique in kidneys.Results: Weight, fasting insulin and insulin sensitivity indexincrease significantly in high fat and sugar feed group, comparedto the group(P<0.01). The weight/kidney weight、24-hoursproteinuria、 serum creatinine and blood urea nitrogen weresignificantly lower and urinary in compared with rats（P<0.05）．IHCdemonstrated that the expression of KLK1in group B，C，D，E weresignificantly higher than that in group A(P<0.01).Compared with thegroup B， the expression of KLK1in group C， D， E wereincreased(P<0.05).Compared with the group C and group D,the KLK1expression level was elevated in group E (P<0.05).it was not foundsignificant difference between the Group C and D (P>0.05).Conclusions: Rats of one model group were intraperitoneallygiven low dose streptozotocin after having the high fat/highsucrose diets can be successful induction type2diabetes mellitusmodel.Combination of benazepril and irbesartan offer futherbenefits arrest proteinuria and protect from renal function andstructure impairment.It is suggested that the expression of KLK1inrenal tissues of rats with diabeticnephropathy is increased than that in normal renal tissue of rats.And blackage of RAS in DN rats with a combination of drugs benazepriland irbesartan increase the expression of KLK1.