| Objective:Designed to investigate the safety of tacrolimus Secretary posterior vitreous injection, while observing the transforming growth factor in rabbit traumatic PVR generate expression when the phase change, and tacrolimus on the expression. To explore the tacrolimus control PVR mechanism of action.Methods:1.Selection of healthy pigmented rabbits were randomly divided into four groups, n=2(4). Each group left eye as the blank drug group Note tacrolimus the Secretary0.1ml concentration of25ug/0.1ml right eye injection of0.1ml saline control group.7,14,21and28days after injection, respectively, slit lamp, direct ophthalmoscope, intraocular pressure (IOP), F-ERG check. Taken at the end of the experiment, the eye HE staining observed by light microscopy. F-ERG b-wave amplitude changes and intraocular pressure by analysis of variance test.2.24healthy rabbits, intravitreal injection of platelet-rich plasma preparation of rabbit PVR model.24rabbits were randomly divided into2groups of12(24eyes), tacrolimus, saline group. Saline group:left eye PVR modeling after30minutes, the vitreous cavity one-time injection of0.1saline; tacrolimus:left eye PVR modeling after30minutes, to be injected into the ball of tacrolimus Division0.1ml (quality The concentration of25ug/0.1ml). Tacrolimus COMPANY rabbit right eye injection of0.1ml saline at the same time as the control group. Each group were7,14,21,28days after the anterior segment and posterior segment examination, reference Fastenberg rating criteria for grading, do eye tissue slices, HE staining, TGF-beta2protein immunohistochemistry staining, and the average optical density of the TGF-beta2positive staining (mean optical density, MOD) to be measured.3.The data were analyzed by SPSS12.0for Windows software package for processing for statistical analysis.Results:1. The eye toxicity observed:Blank drug group tacrolimus injection Secretary the0.1ml (25ug/0.1ml) rabbit eye slit lamp and direct ophthalmoscope not found the rabbit anterior segment and posterior segment of adverse reactions. IOP at each time point with the control group was not statistically significant (P>0.05). ERG b-wave amplitude at each time point comparison with the control group (p>0.05) was not statistically significant. Layers of the retinal histology observed no pathological changes.2.The intravitreal injection tacrolimus Division suppressing observed PVR experimental to:1) blank group rabbit in front of the posterior segment is no exception, before the degree of proliferation and retinal detachment of the vitreous cavity and retina of the saline group rate gradually increased over time, tacrolimus groupcontrast, at7days was not statistically significant (P>0.05), saline group degree of proliferation of the remaining three time points higher than tacrolimus was statistically significant (P<0.05).2) blank group B of the eye ultrasound showed retinal flat cover, no abnormal echo in the vitreous cavity, compared with the saline group, at the same time tacrolimus Division group the vitreous cavity muddy surface of the retina lower degree of proliferation.3) blank retinal layers of normal structure of HE staining, compared with the saline group, tacrolimus company internal limiting membrane surface proliferation and ganglion cell edema missing alleviate.4) blank group Immunohistochemical detection of retinal see a small amount of TGF-beta2-specific staining, tacrolimus Secretary retinal TGF-beta2expression was significantly decreased TGF-beta2expression between the two groups at the same time points, compared with the saline group were statistically learning differences (P<0.05).Conclusion:1.The present experimental study found that normal rabbits intravitreal injection of tacrolimus (25ug) no significant side effects, suggesting that rabbit intravitreal injection of tacrolimus (25ug) in a safe range.2. In this study, platelet-rich plasma intravitreal injection rabbit traumatic PVR model successfully established, and confirmed that the model local retina and hyperplasia, the expression of TGF-beta2abnormally high.3. Tacrolimus can reduce the expression of TGF-β2in the rabbit eye retinal tissue, which in turn has to some extent played inhibit or slow down the PVR-forming role. |
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