Comparative Study Of Surface Plasmon Resonance And Hybrid Capture â…¡ Technology On The Detection Of Cervical Human Papillomavirus

Objective To detect cervical Human Papillomavirus by using surface plasmon resonance technology and hybrid capture Ⅱ technology, explore their value in screening and diagnosing of cervical Lesions.Methods1200patients who had cervical cancer screening were chose from October2010to December2011, Liquid-based cytology test, surface plasmon resonance technology and hybrid capture Ⅱ technology were used to detect high-risk Human Papillomavirus. Cervical biopsy by colposcope were operated on patients with any positive results. The results were compared and analysed.Results1. Detection rate of high-risk Human Papillomavirus was18.75%in normal group,44.73%in CIN Ⅰ group,68.18%in CIN Ⅱ/Ⅲgroup, and92.86%in ICC group, respectively. Compared with normal group, Detection rate in CIN I, CIN Ⅱ/Ⅲand ICC groups were significant higher (P<0.05).2. Sensitivity, specificity, positive predictive value and negative predictive value of two technology are (91.25%、72.50%;50.00%、67.14%;67.59%、71.60%;83.33%、68.12%) respectively in CINⅡ/Ⅲand ICC groups. Surface plasmon resonance technology is of higher specificity and lower sensitivity, statistically significant (P<0.05).3. The total kappa value is0.684, and0.510,0.439,0.604in normal group, CIN I group, CIN Ⅱ/Ⅲand ICC groups respectively(P<0.05).4. The coincidence rate of hybrid capture Ⅱ technology and pathological examination is72.0%, kappa value is0.623, p<0.001. The coincidence rate of Surface plasmon resonance technology and pathological examination is70.0%, kappa value is0.597, p<0.001.5. The main types of high-risk Human Papillomavirus were type16(37.33%), type58(14.67%) type52(10.00%).6. The multiple infection rate in CINⅡ/Ⅲand ICC groups are higher than other groups, but has not statistically significant (p<0.05).Conclusions1. Surface plasmon resonance technology is effective in the detection of cervical Human Papillomavirus.2. The main types of high-risk Human Papillomavirus in china were type16, type58and type52.3. No correlation was found between multiple infection of Human Papillomavirus and cervical lesions.