Effects Of Exogenous Hydrogen Sulfide On Plasma Lipid Level In Hamsters Fed On High Fat Diet And Its Preliminary Mechanism

ObjectiveTo investigate the effects of exogenous hydrogen sulfide on plasma lipidlevel in hamsters fed on high fat diet and to explore the involvedpreliminary mechanism.Methods60male hamsters were randomly divided into five groups: control group;model group (high fat diet, HFD); HFD+NaHS (10μmol/kg/day) group;HFD+NaHS (30μmol/kg/day) group; HFD+NaHS (100μmol/kg/day)group. The HFD+NaHS group were fed with high fat diet and injectedintraperitoneally with NaHS at different concentrations (10,30, and100μmol/kg/day), the control group and model group were injectedintraperitoneally with saline. After3weeks, the plasma total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C), the plasma levels of ALTand AST were measured by automatic biochemistry analyser. The livermorphology was visualized by HE staining, Lipid accumulation in the liver is evaluated by Oil red O staining. The liver MDA content and SODactivity were also determined. The liver LDLR and ABCA1proteinexpression were assessed by western blot.Results1. NaHS reduced plasma lipid level in HFD hamsters. In HFD group, TC,LDL and HDL-C were increased significantly (P<0.05), but not TG,compared with control group. Plasma TC, LDL, HDL-C level weresignificantly decreased (P<0.05) in HFD+30μmol NaHS group andHFD+100μmol NaHS group, and plasma TG level was significantlydecreased in HFD+10μmol NaHS group and HFD+30μmol NaHS group(P<0.05), compared with HFD group.2. NaHS inhibited the damages of liver in HFD hamsters. In general, thesurface of liver is smoothly, dark red, bright luster, sharp edges, flexibletexture in normal hamster. In HFD group, the livers of hamsters isyellowish-white on the whole, diffuse enlargement, capsular tension,edge blunt and thick, soft texture, trimming has a greasy feeling. Themorphology of livers in NaHS treatment groups were significantlyimproved compared with HFD group. In microscopic morphology,disorganized liver tissue, unclear structure of hepatic lobule, andcompletely disappear liver sinus were significantly ameliorated in NaHSintervention group, compared with HFD group. At the same time, thesteatosis was significantly improved in HFD group than NaHS intervention group.3. NaHS inhibited the lipid accumulation of liver in hamster fed on highfat. Oil red O staining showed that HFD consumption increased liver lipidcontent, which was significantly attenuated by NaHS treatment.Meanwhile, the liver weight of HFD groups was significantly higher(P<0.05) than that of control group, showed as relative liver weight,and compared to HFD group, the liver weight/body weight ratio ofHFD+30μmol NaHS group and HFD+100μmol NaHS group weresignificantly decreased (P <0.05).4. NaHS inhibited plasma ALT, AST levels in HFD hamsters. PlasmaAST, ALT in HFD group was obviously increased (P<0.05) in HFDgroup, compared with control group, plasma AST, ALT was significantlydecreased (P<0.05) in HFD+30μmol NaHS group and HFD+100μmolNaHS group, compared with the HFD group.5. NaHS decreased MDA content and increased SOD activity in HFDhamster’s liver. Compared with control group, MDA content increasedand SOD activity decreased significantly (P<0.05) in HFD group hamster.Compared with HFD group, MDA content decreased and SOD activityincreased in different concentration of NaHS intervention group.6. NaHS increased concentration-dependently the liver LDLR proteinexpression compared with HFD hamster, without affecting the expressionof ABCA1protein. Compared with the control group, LDLR protein expression of the HFD group liver decreased (P<0.05).Conclusions1. Blood lipid level in hamster fed a high fat diet can be reduced byexogenous hydrogen sulfide.2. Exogenous hydrogen sulfide decreases blood lipid level throughprotecting liver and increasing the liver LDLR expression.