Investigation On The Effection And Mechanism Of Lower Limb Ischemia Perconditioning On Myocardial NR After IR

Objective lower limb ischemia perconditioning intervens SD rats withischemia-reperfusion, to observe the impact of the no-reflow phenomenon and studythe role of the inflammatory response in the no-reflow phenomenon。Methods4-month-old、350-450g、36SD rats were randomly divided into3groups:sham operation group、 IR group and LLIPerC group。 Rats wereanaesthetized with pentobarbital sodium and trachea intubation for assistedbreathing。 After60-min of left anterior descending coronary artery (LADCA)occlusion followed by90-min of reperfusion, Evan’s blue distinguishs betweenischemic area and non-ischemic area， myocardial NR area was measured byThinflavin-S； myocardial infarct size was measured by TTC； BL-420detectscardiac function； Tissue sections、 malondialdehyde(MDA)、 IL-6andmyeloperoxidase(MPO) reflect inflammation。Results Hemodynamic parameters、 Myocardial infarct size、 aggregation ofneutrophils、IL-6、MDA and MPO of LLIPerC and sham groups were significantlyless than IR group（P＜0.05），there was different in aggregation of neutrophils、myocardial infarct size、NR area、IL-6、MDA and MPO of LLIPerC and shamgroups(P＜0.05)。Conclution LLIPerC group significantly reduce myocardial infarct size、aggregation of neutrophils、releasing of IL-6、MDA and MPO。LLIPerC producescardioprotection in IR injury by reducing the inflammatory response。...