The Novel Methods For The Determination Of Phenol Mercury Niteite And Styrene In Environmental

In the chapter1, the significance of study and headways trend of the laboratorytechnologies for phenol,mercury,niteite and styrene were introducted respectively.In the chapter2, a fluorescence quenching spectrometry new method forhigh-sensitivity determination of phenol was established.The characteristics of theultraviolet absorption spectra and fluorescence spectra were studied.The affectingfactors on the strength of fluorescence were investigated including acidity, dosage,temperature, reaction time, coexisting ions and the optimum conditions of the reactionin the system. In HAc buffer solution system, the results obtained showed that thedecrement of fluorescence quenching was proportional to the concentration of phenolin the range of4.36×10-3μg/mL～0.35μg/mL,the linear regression equation was ΔF=36.91+309.56ρ（ρ: μg/mL） and detection limit was1.30×10-3μg/mL. when theproposed was used to determine trace phenol in water samples, the recoveries was96.0%～104.5%.In the chapter3, based on fluorescence quenching of2-PBI by mercury ion, anew method of inhibition fluorescence has been developed for determition of mercuryion. The characteristics of the ultraviolet absorption spectra and fluorescence spectrawere studied; The affecting factors and the optimum conditions of the reaction in thesystem were investigated. under the optimized test conditions. There was a linearrelationship between the decrease intensity of fluorescence（ΔF） and the concentrationof mercury nitrate in the range of8.27×10-8⁴.0×10-6and the linear regressionequation was ΔF=125.44c+16.75（c for mercury nitrate concentrations,×10-6mol/L）, correlation coefficient r=0.998, the detection limit was2.41×10-8mol/L.Thismethod was sensitive,simple,easy to operation, good precision. It was satisfactoryresults for the determination of mercury in the environment.In the chapter4, a reversed phase high performance liquid chromatographicmethod for the determination of Niteite was developed, based on fluorescence quenching of AR by Niteite. Using HPLC-fluorescence detector to quantify anddetermine Niteite. Choosing the optimized chromatographic conditions, the systemwas separated on a Agilent TC-C18（5μm,150×4.6nm） chromatography column anddetected at λ_ex=530nm, λ_em=550nm by using methanol-acetonitrile-（0.1%） TDA（50∶25∶25v/v） as mobile phase when the column temperature was30℃and theflow rate was1.0mL/min. The calibration curves of styrene showed good linearity inthe ranges of10.0ng/mL～400.0ng/mL, the linear regression equation wasΔU=108.72ρ+70.58（r=0.998）,the detection limit for was1.5ng/mL, P=93.3%～106.8%.In the chapter5, a reversed phase high performance liquid chromatographicmethod for the determination of residue styrene in disposable lunch boxes wasdeveloped. And its dissolving characteristics were analysed in the five in the extractsolutions. Choosing the optimized chromatographic conditions, styrene was separatedon a Agilent TC—C18（5μm,150×4.6nm） chromatography column and detected at245nm by using methanol in water （90∶10, v/v）) as mobile phase when the columntemperature was45℃and the flow rate was1.0mL/min. The calibration curves ofstyrene showed good linearity in the ranges of0.05～90.00μg/mL, the linearregression equation was A=2.45ρ－0.18（r=0.9997）, the detection limit for was0.03μg/mL. P=92.00%～107.00%. The proposed method was specific, sensitive,accurate and suitable for the quantification of residue styrene in tableware.