The Express And Significance Of E-cadherin In Primary Nephrotic Syndrom Patients’ Podocyte

Objective：E-cadherin (E-cadherin) is a kind of important calcium dependenceadhesion molecules，It plays an important role of happening on embryo development,morphology and maintaining adult tissues epithelium polarity and integrity,Participate in building and maintaining a connection between the normal cells.Because of its reduced in renal tissue, lead to glomerular podocyte and basementmembrane connection damage, make podocyte from off glomerular basementmembrane. The experiment detects kidney tissue E-cadherin expression in primarynephrotic syndrome (primary nephrotic syndrome,PNS) patients，discuss the role ofthe podocyte damage.Methods: Case group:30patients with primary nephrotic syndrome admitted into thishospital from Jun.2010to Jun.2012aged within20-60years were recruited, after therenal puncture biopsy, their pathological types were diagnosed as mild mesangialproliferative glomerulonephritis. Control group:20cases of normal kidney tissuessampled in surgery of kidney tumors by resecting normal tissues at more than2cmaway from tumor margin. Detects blood biochemical indicators(albumin value,creatinine, urea value, cholesterol, triglycerides,LDL), returns24hours urine test for24hours urinary protein quantitative value;HE、Masson、PAS dyed observates twogroups of renal tissue general pathological changes, observates glomerular mesangialcell proliferation and renal interstitial fibrosis degree,grade renal interstitial fibrosislevel; Immunohistochemical detectes E-cadherin expression in glomerular podocyte,Immunofluorescence detectes Nephrin expression in glomerular podocyte. discuss thecorrelation between E-cadherin expression in renal tubular and renal interstitialfibrosis,also discuss the the correlation between Nephrin expression and24hoursurinary protein quantitative. discuss the function of the both in podocyte injury andproteinuria produce process.Results:1. In general:17were male,13were female in experimental group, averageage45.12±9.27, creatinine value:64.41±16.29umol/L, urea value:4.95±2.04mmol/L,albumin value:22.13±4.56g/L,24hours urinary protein quantitative:3.48±0.49g/24H,cholesterol:7.47±2.05mmol/L,triglycerides:2.50±0.44mmol/L,LDL:3.71±0.74mmol/L;The control group:12were male,8were female, average age:49.90±10.36, creatininevalue:63.89±12.34umol/L,urea:7.16±2.62mmol/L,albuminvalue:40.97±5.69g/L,cholesterol:5.27±1.09mmol/L,triglycerides:1.99±0.32mmol/L,LDL:2.69±0.41mmol/L;2.Pathological dyeing: Compared with controls, diffuse glomerular mesangial cellproliferation in PNS group, mesangial matrix increase,renal interstitial mild broadening,part of the mild fibrosis, inflammatory cells increased;3.E-cadherinexpression: E-cadherin no expresses in both of PNS group and control group patients’glomerular podocyte, Compared with the control, E-cadherin expression in therenal tubular of PNS groups is reduced（P<0.05）;4. Nephrin expression: Comparedwith the control, Nephrin expression in the renal tubular of PNS groups is reduced（P<0.05）.5. There is no correlation between E-cadherin expression in renal tubularand renal interstitial fibrosis; there is negative correlation between Nephrin expressionand24hours urinary protein quantitative.Conclusion:1. The E-cadherin is not expressed in the podocytes of the PNS patientswith pathology type of mild mesangial proliferative glomerulonephritis, and it is notinvolved in the podocyte injury (shedding).2. The E-cadherin is an important factorthat leads to tubulointerstitial damage, and it is involved in the trans-differentiationprocess of the tubular epithelial cells.3. The Nephrin is involved in the generation ofproteinuria and negative correlation between Nephrin expression and24hours urinaryprotein quantitative.