HHV-8Viral Load Associated With Kaposi’s Sarcoma

Objective: Detection of kaposi’s sarcoma (KS) and Control group HHV-8DNA copy number;Analysis of two groups of samples, the HHV-8load difference;Analysis of different stages oflesions KS about the difference of HHV-8load.Discusses significance of HHV-8loads in KSpathogenesis.Methods: Division KS paraffin specimens nodules, plaques,,plaque of20patients and SCC6cases,and were extracted genomic DNA for HHV-8open reading frame (ORF)26PCRamplification and ORF26plasmid standard,the use of real-time fluorescent quantitative PCRmethod to detect the viral load in KS and SCC.Results:HHV-8ORF26objective fragment was successfully prepared, and to obtain a stablerecombinant plasmid, to maintain the specificity and sequence integrity of the target fragment, agood standard curve parameters, a good linear relationship ORF26standard CT value and itscorresponding gradient dillution standard concentration, The amplification efficiency of89.916%. Successful detection of Kaposi’s sarcoma and squamous cell carcinoma in viral load.Althoughthere are very significant differences detected in KS and SCC was no significant difference in theviral load （t=1.794,P=0.087）,P>0.05. Analysis of Kaposi’s sarcoma clinical phenotype of viralload comparison (F=1.399, P=0.274) was not statistically significant.Conclusions:There is unrelated in HHV-8load and KS clinical stage.HHV-8DNA copy numberin tissue samples KS is very low, suggesting that HHV-8in the pathogenesis of KS proliferationdoes not depend on the speed of the disease, but the virus itself genes encoding homologs of thevarious genes involved in disease.