Fluorescence Quantitative Analysis Of Pefloxacin Mesylate In Body Fluids With Second-order Calibration

With the rapid development of modern science and technology, the natural phenomenahave been studied in depth. The application systems that chemistry researchers are facedbecome more and more complicated, which makes physical scientist manufacture a largenumber of multi-channel and high-order analytical instruments. The data produced by theinstruments are three-dimensional or four-dimensional data arrays, rather than just simplescaler or vector response data. Development of chemometrics theoretic can provide variousefficaciously methods for extracting useful chemical information from these complexity dataarrays.Pefloxacin mesylate is a new drug of fluorinated quinolone antibacterial, On G-and G+bacteria, including Enterobacteriaceae, Pseudomonas aeruginosa, Acinetobacter sp.,Haemophilus, Neisseria spp. and Staphylococcus aureus (including methicillin-resistantstrains) with broad-spectrum activity. The resistance of Staphylococcus aureus andvancomycin is similar, but the resistance of Pseudomonas aeruginosa than ciprofloxacin andceftazidime, also effective to some multivalent resistant strains and methicillin resistantbacteria. In oral and intravenous injection conditions, the rate of about30%of pefloxacinmesylate protein binding.Pefloxacin mesylate in vivo metabolic activity product ofdemethylation derivatives and no activity N-oxide, t1/2in10-12hours.Pefloxacin mesylategood absorption in the gastrointestinal tract, after oral administration the area under theconcentration-time curve and similar to the intravenous administration. Pefloxacin mesylate iswidely distributed in the body, cerebrospinal fluid, tonsil, bronchial, bone and muscle,prostate and peritoneal fluid reach the effective concentration.That mainly through thekidneys and liver to eliminate about50%. Therefore, pefloxacin mesylate in plasma and urinequantitative analysis is very important. The research work in this thesis mainly focuses onrapid and green anlaysis of Pefloxacin mesylate in body fluids. The second-order calibrationmethods combined with the excitation-emission matrix fluorescence based on enhance weredeveloped to analyze Pefloxacin mesylate in human plasma samples and urine samples. Studyworks presented in the thesis primarily deal with the following aspects:1. Construction of analysis model for Pefloxacin mesylate with the second-order calibrationmethods (Chapter2)Pefloxacin mesylate is a kind of new-4-methyl piperazine quinolone derivatives, it caninhibit DNA gyrase blocking DNA replication and exert their antibacterial effects. At present, main methods for determining of pefloxacin mesylate in biological fluids were: Highperformance liquid chromatography, Capillary electrophoresis electrochemiluminescenceassay, Flow injection chemiluminescence method etc. Second-order calibration based onparallel factor analysis (PARAFAC), alternating trilinear decomposition(ATLD),self-weightedalternating trilinear decomposition(SWATLD)combeined with micellar enhanced excitation-emission matrix fluorescence for determination of pefloxacin mesylate.Satisfactory resultsrevealed that the methods were simple, rapid, sensitive and reliable to be handled to determinepefloxacin mesylate.2. Quantitative analysis of Pefloxacin Mesylate in plasma with Second-order calibrationmethod (Chapter3)Rapid drug analysis in complex body fluid is an important problem in modernbiomedicine, the traditional method using chromatographic separation technology to achievethis objective. Usually by adjusting column chromatographic or separation conditions, butbecause plasma contains unpredictable matrix and other substances, and in some cases areoften difficult to achieve complete separation, and for the effects of extraction separationcondition is also very time-consuming consumables. Therefore, considering the fluorescencedetection has high sensitivity, high selectivity, low cost, and the stoichiometry of second-ordercalibration methods to the first-order calibration methods with Second-order advantage: evenwith unknown interference coexistence can also are interested in quantitative analysis. In thispaper, using the second-order calibration methods based on parallel factor analysis,alternating trilinear decomposition, self-weighted alternating trilinear decomposition,combined with three-dimensional excitation-emission fluorescence spectrum, analysis ofpefloxacin mesylate three-dimensional fluorescence spectra in plasma. In pefloxacin mesylateand plasma, overlapping response signal interference case, removal of the signal of unknowninterferents response in plasma samples using "mathematical separation", as well asquantitative blood concentration of simple regression method realization of pefloxacinmesylate direct selective analysis, obtain quantitative results. The method is simple, cost islow, quantitative results are satisfactory. Not only fluorescence as a target group of weakfluorescent or non-fluorescent complex system, the analysis provides an effective andconvenient way, but also has the potential to become a clinical monitoring in body fluid ofpefloxacin mesylate was rapid, sensitive analysis method.3. Quantitative analysis of Pefloxacin Mesylate in urine with Second-order calibration method(Chapter4) Through the three-dimensional fluorescence technique combined with parallel factoranalysis, alternating trilinear decomposition, self-weighted alternating trilinear decomposition,"Mathematical separation" instead of "physical and chemical separation", and with the help of" Second-order advantage" second-order calibration methods, the successful implementation ofthe content of high sensitive, fast, with green direct selective determination of urine samplesof pefloxacin mesylate. For the future of pefloxacin mesylate dose recovery study, researchand determination of metabolite type rate and bioavailability of renal clearance, to provide arapid, simple analysis method.4. Quantitative analysis of pefloxacin mesylate in matrix model with second-order calibration(Chapter5)This chapter studies the second-order calibration method in the same day and differentdays of matrix models was constructed for the prediction of body fluid samples of pefloxacinmesylate analysis ability. Through the evaluation on the quality factor, including selective(SEL), sensitivity (SEN), limit of detection (LOD) and test (LOQ). The results show that thesecond-order calibration method can be in a variety of human fluid samples for simultaneousdetermination of some component of interest, to maintain and develop the "second-ordercalibration" play in human body fluid samples of drug concentration in the role of, it willmake to further enrich the content of second-order calibration method. This method not onlyprovides a high sensitive method for complex fluids green drug concentration in the study, isalso expected to study pharmacokinetics in vivo drug provides a more reliable method,simple.