Endothelial Progenitor Cells Transplantation From Myocardial Infarction And The Effect Of Fasudil Hydrochloride On The Heart Function After Myocardial Infarction

Objective To observe the effect of Fasudil hydrochloride on human EPCs in vitroculture and on cardac fuction of rat model of myocardial infarction after transplantationof EPCs in rats, and to dicuss its mechanism. Methods （1）we extract peripheral bloodrespectively from the healthy volunteers and patients with acute myocardial infarction(within12h) by differential attachment for extracting mononuclear cells and culturingendothelial progenitor cells, in order to observe the differentiation, proliferation,apoptosis and angiogenesis ability of EPCs, and to observe the effect of Fasudilhydrochloride (HF) on culturing EPCs in vitro;(2)60SD rats were selected forpreparation of myocardial infarction model, and we devided the success models into4groups:①Control group, we injected normal saline into abnormal cavity;②HFgroup,we injected HF10mg/kg into abnormal cavity;③EPCs transplantation group, transplantEPCs into myocardial;④EPCs transplantation+HF group, we transplant EPCs intointramyocardial and injected HF10mg/kg into abnormal cavity and kept for14days.We cultured the EPCs, which would be transplanted into intromyocardial of rats, fromperipheral blood of small rats with the knockout of β-galactose glucoside enzyme gene,and after transplantation, we identified the EPCs with β-galactose glucoside enzyme.Finally, we can detect the regional myocardial blood flow, the vascular density and thedegree of firosis in each group.Results⑴we successful cultured and obtained the EPCsof peripheral blood from the patients with acute myocardial infarction by differentialvelocity adherent screening method.After cultured with HF, the ability of celldifferentiation, proliferation and blood vessel formation significantly higher than the HFgroup(p＜0.05), while the cell apoptosis was lower than the group withou HF (p＜0.05);⑵In EPCs transplantation+HF group, the myocardial blood flow and vascular densityincreased, the degree of myocardial fibrosis reduced, Left ventricular end-diastolicdiameter (LVEDD) and systolic end inside diameter (LVESD)has significantly reduced (p<0.05), the ejection fraction (EF) increased and heart function has improved.Conclusions⑴Using the differential adherence screening method, we has extractedthe mononuclear cells, and successful extracted and cultured EPCs in different periods.⑵Culturing EPCs from patiens with myocardial infarction in vitro added Fasudilhydrochloride,which can promote cell differentiation, proliferation, reduce apoptosis andincrease the ability of blood vessel formation.⑶After transplantation EPCs into rats,adding HF can increase the regional myocardial blood flow, vascular density, reduce thedegree of myocardial fibrosis, and improve heart function;⑷The effect of HF for EPCsin vitro culture and in vivo transplantation of EPCs experiment, we can figure out thatHF,as Rho kinase inhibitors, possibly through inhibiting the expression of Rho kinase inendothelial cells, improve the activity of eNOS, and assist in improvingmicroenvironment after transplantation for myocardial infarction EPCs.Makes EPCstransplantation be better survival and differentiation. And makes EPCs are better able torepair damaged myocardial cells, promote angiogenesis and improve myocardial bloodsupply, reduced infarct size, limit ventricular remodeling. These provide the experimentalbasis for myocardial infarction in patients with EPCs transplantation combined with HFtreatment.