| Objective To investigate the effects of RELMβ transfection on the proliferation,migration and angiogenesis of human umbilical vein endothelial cell.Methods Under the mediation of liposome, the RELMβ expression vector wastransfected into human umbilical vein endothelial HUVEC cells, which weredivided into three groups, including blank control group, vector group, andRELMβ group. Cell proliferation was measured by MTT colorimetric andEdU incorporation assays. The migration and angiogenesis of HUVEC cellswas detected by transwell and tube formation assay, respectively.Results In HUVEC cell line, no significant difference was observed between controland vector groups in proliferation rates, migration rates and angiogenesiscapacity. The proliferation rate of HUVEC cells in RELMβ group wasdecreased by48.3%(P <0.05) via MTT assay, and was significantly reducedby25%(P <0.05) via EdU assay. Transwell assay showed that the migrationof RELMβ-transfected HUVEC cells was decreased by65%(P <0.05), whiletube formation assay indicated that the angiogenesis of RELMβ groupdecreased significantly compared with control group, with smaller diameterand low ring rate.Conclusion Transfection of RELMβ inhibites the proliferation, migration and angiogenesisof human umbilical vein endothelial cell. Objective To investigate the effects of recombinant RELMβ protein on the proliferation,migration and angiogenesis of human umbilical vein endothelial cell.Methods Recombinant RELMβ protein was administrated to human umbilical veinendothelial cell lines HUVEC, which were divided into five groups, includingblank control group, solvent (BSA) group and recombinant RELMβ proteingroup (10ng/ml,50ng/ml and100ng/ml). Cell proliferation was measuredby MTT colorimetric and EdU incorporation assays. The migration andangiogenesis of HUVEC cells was detected by transwell and tube formationassay, respectively.Results In HUVEC cell line, no significant difference was observed between controlandBSA groups in proliferation rates, migration rates and angiogenesiscapacity. The proliferation rate of HUVEC cells treated by RELMβ protein(10ng/ml,50ng/ml and100ng/ml) was was decreased by8%(P>0.05),19.3%(P<0.05) and22.5%(P<0.05) via MTT assay, and was significantlyreduced by16.4%(P<0.05),23.3%(P<0.05) and25.4%(P<0.05) via EdUassay. Transwell assay showed that the migration of RELMβ-transfectedHUVEC cells was decreased by13.5%(P<0.05),34.6%(P<0.05),56.5%(P<0.05), while tube formation assay indicated that the angiogenesis ofRELMβ group decreased significantly compared with control group, withsmaller diameter and low ring rate.Conclusion Recombinant RELMβ protein inhibits the proliferation, migration andangiogenesis of human umbilical vein endothelial cells. Objective To explore the mechanism underlying the inhibitory effects of RELMβ on the angiogenesis of human umbilical vein endothelial cells.Methods Recombinant RELMβ protein was administrated to human umbilical veinendothelial cell lines HUVEC, which were divided into five groups,including blank control group, solvent (BSA) group and recombinantRELMβ protein group (10ng/ml,50ng/ml and100ng/ml). Wersten blot andreal-time PCR were applied to meature the expression of VEGF and itsreceptors.Results In HUVEC cells, no significant difference was observed between control andBSA groups in expression of VEGF and its receptor KDR and FLT-1. Westernblot indicated that administration of recombinant RELMβ protein (10ng/ml,50ng/ml and100ng/ml) resulted in decreased VEGF expression by11.8%(P<0.05),41.6%(P<0.05),67.2%(P<0.05). Real-time PCR indicated that theexpression of KDR was increased by3.05times (P>0.05).1.5times（P<0.05）and1.42times(P>0.05), while the receptor KLT-1was increased by4.3times(P>0.05).1.63times (P<0.05) and1.75times (P>0.05).Conclusion RELMβ can down-regulate the expression of VEGF in human umbilical veinendothelial cells, but up-regulate the expression of VEGF receptors, KDRand FLT-1. The exact mechanisms warrat further investigation. |
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