Ethyl Pyruvate Protection Against Hydrogen Peroxide Toxicity In Human SH-SY5Y Cells

Objectives：Observe the effects of hydrogen peroxide on the cell viability.To evaluate theprotective effects of Ethyl pyruvate on the SH-SY5Y cells induced by H2O2and explore thepossible mechanism of this protective effect.Materials and Methods: The MTT method was used to evaluate the cell viabilities ofhuman neuroblastoma SH-SY5Y cells, and immunofluorescent cell staining technique wasemployed to evaluate the ROS levels in cells.The western blotting was used to evaluate theintracellular Bcl-2and Bax. Finally, staining with Hoechst33258, to observate themorphological changes of the nucleus in treated and untreated group separately.Results: The results showed that H2O2decreased the cell viability of SH-SY5Y cells.Ethyl pyruvate can effectively improve such damage. Intracellular ROS levels detected incells treated with hydrogen peroxide was significantly increased, but ethyl pyruvate EP cansignificantly reduce this oxidation reaction. Western blotting found H2O2treated groupBcl-2decreased and Bax increased. EP group Bcl-2and Bax changed to the contrary. Theimmunofluorescence results found, in hydrogen peroxide treated cells, stained nuclearsignificantly reduced.Compared with the control group,part of the cells appeard nucleushighly-stained, fragmentation.Conclusions: Hydrogen peroxide could greatly promote the intracellular reactive oxygenspecies, indicating a vital role of oxidative stress in H2O2induced cell damage; EP coulddecrease intracellular oxidative stress levels and lower apoptotic proteins to protect cellsinduced by H2O2, showed an important neuroprotective effect against oxidative damage.