Study On Radiosensitization Of Docetaxel-loaded PLGA Nanoparticles In CNE-1and A549Cells In Vitro

【Objective】To fabricate Docetaxel-loaded PLGA nanoparticles by the optimizednanoprecipitation method and compare the cytotoxicity and radiosensitivity ofnanoparticles with those of Taxtere on CNE-1and A549cells in vitro.【Methods】The nanoparticles were fabricated by optimized nanoprecipitation method andcharacterized by DLS, Zeta potential, FESEM, HPLC. The cell viability was detected byMTT assay. Flow cytometry was used to quantify the rate of tumor cells held in the G2/Mphase in cell cycle. Radiosensitivity of Taxtere and drug-loaded nanoparticles wasinvestigated by colony forming assay on CNE-1and A549cells.【Results】The fabricated nanoparticles were spherical in shape with size between130nmand150nm. The encapsulation efficiency was40.83.【Conclusion】The present research proposed that the TPGS emulsified docetaxel-loadednanoparticles fabricated by nanoprecipitation technique can be utilized to enhance theradiosensitivity of cells resistant to radiation such as A549and CNE-1cell lines. Thisnanoparticle system is demonstrated as prospective carrier for docetaxel and showspotential for the future in vivo development of a radiosensitizer delivery system.