Effect Of Gypenosides On The Cognitive Function And The Change About PCREB And BDNF In The Hippocampus Tissue Of Mice With Vascular Dementia

0bjective: To investigate the influence of Gypenosides on the cognitive function and thechange about phosphorylation cAMP-response element binding protein(pCREB) andbrain-derived neurotrophic factor(BDNF) in the hippocampus tissue of mice with vasculardementia (VD)，and explore the probable mechanisms．Methods:The models of VD mice were established through three timesischemia—reperfusion of bilateral common carotid arteries．The male mices of C57BL/6Grandomly divided into the sham operated group，vascular dementia model group，andGypenosides dispose groups(low，middle and high dose group)．The ability of spaciallearning and memory of vascular dementia mices was tested by Morris water maze．Thechange about the neuronal injury in the mice hippocampal CA1region was detected by HEstaining．The expression of phosphorylation cAMP-response element binding proteinmRNA and brain-derived neurotrophic factor mRNA was observed by Real time PCR．Results:①Morris water maze test found that the ability of learing and memory of VDmice decreased．The escape latency of VD group mice was obviously longer than that insham group(P<0.05)；The escape latency in GP low，medium and high dose group micewas even obviously shorter than that in VD group(P<0.05)；The escape latency betweenGP low，medium and high dose group had no significant difference(P>0.05)；Through theoriginal platform time of VD group mice was markedly longer than that in shamgroup(P<0.01)；Through the original platform time of GP low，medium and high dosegroup mice was shorter than that in VD group(P<0.05)；Through the original platform timeof GP high dose group mice was shorter than that in GP medium dose group(P<0.05)；Through the original platform time between GP medium and low dose group had nodifference(P>0.05)．②HE staining showed that the hippocampus CA1area neurons cellulaform of sham group mice was mormal after72h，the structare was full，the number wasmany，the cell arranged closely and neatly，the nuclei was round or elliptic；VD group hippocampal neurons cell appeared abundant patchy necrosis，the cell number wasdecreased obviously， the arrangement was loose， the nucleus was hyperchromaticpycnosis．The hippocampal neurons cell number of GP low dose group was slightly more，the level arranged neatly．The hippocampus CA1area neurons cellula number of GPmedium and high dose group increased，the obvious patchy necrosis was rare，the cellulastructure was quite clear，the arrangement was obvious neat，the cell nucleus pycnosis wasrare．③Compared with sham group， the expression of VD group hippocampuspCREBmRNA and BDNFmRNA even decreased clearly in24h and72h(P<0.05)；Compared with VD group，the expression of GP low，medium and high dose grouphippocampus pCREBmRNA and BDNFmRNA even increased significantly(P<0.05)．Conclution: Cerebral ischemia reperfusion injury can lead to vascular dementia micehippocampus neuron damage and the decreased ability of spacial learning andmemory．Gypenosides can reduce the neuronal injury and increase the expression ofpCREBmRNA and BDNFmRNA in the hippocampus of VD mice．It make for improvingthe ability of spacial learning and memory in the VD mice．...