Angiogenesis Inhibited By HIF-1α Gene Silencing In Oral Squamous Cell Cancer:in Vivo

Objective To investigate the role of HIF-1α in angiogenesis in KB oral carcinoma murinexenograft model.Methods1.Constructing HIF-1α shRNAto target human HIF-1α in KB cell, Western blotassay and Real time reverse transcriptase polymerase chain reaction(Real time RT-PCR) wereused to screen the vector HIF-1α-1with a highest inhibitory rate.2.We injected selected stablyHIF-1α silenced and untreated KB cell into two different groups:interference group andcontrol group to establish murine xenograft model,observe growth state and drawing growthcurve.3.Using HE staining and CD31immunohistochemical staining to observe themorphological change of blood vessel and microvessel density(MVD).3. Detect theexpression of HIF-1α downstream target genes VEGF、TGF-β1、OPN and EGFR byimmunohistochemical staining.Results1.Growth curve shows that the growth speed and tumor volume of interference groupare significant slower and smaller than control group(P<0.05).2.Compared with control group,the morphology of blood vessel in interference group is verging to normal and the MVDreduced significantly(P<0.05).3.The expression of VEGF、TGF-β1、OPN and EGFR ofinterference group is remarkable weaker than the control group.Conclusions Silencing HIF-1α can inhibit the expressions of VEGF、TGF-β1、OPN andEGFR, makes the structure and morphology of tumor vessel normalized,the amount of vesselreduced,the growth of tumor delayed.