| Objective: Our aim was to explore the mechanism underlying the transcriptionalregulation of microRNA-199a/214in Rat myocardiocyte H9c2cells.Methods and results: Using Co-Immunoprecipitation and Farwestern blot wedemonstrated that PARP-1can combines with Twist-1, and Twist-1waspoly(ADP-ribosyl)ated in cultured myocardiocyte H9c2cell. Using real-time RT-PCR assay,the expression of microRNA-199a/214was determined. The DNA binging activity wastested by using electrophoretic mobility shift assay. Our results showed that inhibition ofpoly(ADP-ribose)polymerse-1(PARP-1) by3-aminobenzamide, PJ34,or PARP-1siRNAmarkedly reduced the transcription of microRNA199a/214in myocardiocyte H9c2cells.Twist-1was poly(ADP-ribosyl)ated by PARP-1in HepG2cells under basal conditions.Inhibition of PARP-1reduced the DNA binding and transactivation of Twist-1and reducedthe transcription of Twist-1-target genes microRNA-199a/214.Conclusion: This study illustrated that Twist-1was poly(ADP-ribosyl)ated by PARP-1,and poly(ADP-ribosyl)ation enhanced the DNA binging of Twist-1and increased thetranscription of Twist-1-target genes microRNA-199a/214. |
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