| BACKGROUNDOsteoarthritis (osteoarthritis, OA) is a common chronic disease whichis the joint inflammatory lesions caused by inflammation, infection,trauma, or other factors,it belongs to rheumatic diseases. OA patients inChina are estimated100million or more, and the number is increasing.According to statistics, half of Chinese people over the age of50,65haveOA. Most of OA is caused because of knee joint illness, pain andrestricted movement of americans associated with knee OA, age of55to64more than13%.More than17%is65to65years old. The social burdenof OA is related with the present form of the spread of OA. It is estimatedthat between25and75Americans, a clinical signs of OA symptomsaccounted for12.1%. Increased along with the age increase, the incidenceof symptomatic OA, coupled with the symptoms of OA improve treatmentis not completely, thereby increasing the overall economic burden of OA.The treatment of OA lacks of effective means of mainly symptomaticto relieve patients’ pain. Main means to have surgery and drug therapy,surgical treatment carries a risk of infection, and drug therapy can relieve pain has no good effect on cartilage degeneration. Hospitalized patientswith main purpose is to fully control the pain and joint maximumprotection function and reduce morbidity, and the pain is the mostcommon osteoarthritis patients seek medical help. Both can relieve painand, therefore, can delay the degeneration and promote the repair ofarticular cartilage becomes the key to treatment. Choose a suitable way totreat arthritis, to relieve symptoms, delay the degeneration of articularcartilage, improve patient’s quality of life, to explore a more effectivetreatment of arthritis, is the urgent desire of many arthritis sufferers, butalso clinical research hotspot nowadays.Ultrasonic drug penetration therapy as a new delivery way becomeshot spots, for our treatment of OA, provides a new treatment. Ultrasonictherapy drug penetration therapy, also known as sound, is to add drugs tothe coupling agent, in under the action of ultrasonic wave, make the drugsthrough the skin or mucous membrane through human skin into the bodyof a treatment. Ultrasonic penetration therapy can avoid oral drugs causeside effects, and glucosamine sulfate to the degeneration of articularcartilage has the effect of delay.Based on the above background, this research use ultrasonictherapeutic apparatus, arthritis by ultrasonic assisted glucosamine sulfatein vitro through rabbit skin, can study of glucosamine sulfate in under theaction of ultrasound through the skin, and ultrasonic parameters is discussed in this paper, and through the relationship between the rate andamount through to ultrasonic percutaneous penetration glucosaminesulfate arthritis to find the best irradiation conditions. And then choose theappropriate ultrasonic irradiation of rabbit knee joint parameters,ultrasonic percutaneous penetration of glucosamine sulfate treatment ofrabbit knee osteoarthritis treatment effect is analyzed.PURPOSE1. Explore the glucosamine sulfate solution can in under the action ofultrasound through in vitro rabbit skin, calculate the rate glucosaminesulfate through and through, discuss the effect of ultrasonic percutaneouspenetration and ultra sound intensity, irradiation time, the relationshipbetween the diffusion liquid concentration and thickness of the skin,ultrasound screening ultrasound assisted transdermal treatment conditionsfor the next step.2. Select the best irradiation parameters of ultrasonic therapeuticirradiation rabbit knee arthritis, obtained after treatment made for grossobservation, articular cartilage pathological examination, after in kneejoint fluid and blood testing the concentration of hyaluronic acid,comparison between groups after the evaluation of treatment effect.Ultrasonology percutaneous penetration glucosamine sulfate as aneffective treatment for osteoarthritis of the articular cartilage degeneration of the method is feasible.METHODS1. In vitro experiment1.1Instruments and equipment: Chongqing melting sea ultrasonic medicalengineering research center co., LTD. Provide the ultrasound therapeuticarthritis (the frequency is1.0MHz, the irradiation area is5cm2).1.2Subjects: Back and side abdominal in vitro rabbit skin.Experimental methods:1.3air embolism executed New Zealand whiterabbit, stripping of rabbit skin, the skin in homemade improvised Franzdiffusion liquid pool, therapeutic arthritis treatment head on grouping inthe spread of glucosamine sulfate liquid ultrasonic irradiation, areaccepted after fluid to carry on the color reaction, to determine whether aglucosamine sulfate through the skin; Configuration of glucosaminesulfate standard solution and through the ultraviolet spectrophotometrydrawing standard curve of glucosamine sulfate solution, based on thestandard curve determination of glucosamine sulfate concentration inaccept night, glucosamine sulfate through and through the rate calculated;Analysis different sound intensity, irradiation time, solution concentrationand the thickness of the skin on the amount of glucosamine sulfate throughand through rate, results application SPSS19.0statistical package andMicrosoft Excel software for analysis. 2. In vivo experiment2.1experimental apparatus: As in vitro experiment.2.2Subjects: New Zealand white rabbits.2.3Experimental methods:40New Zealand white rabbit to select righthind legs, according to the improved Hulth method in animal surgicalremoval of the inside of the knee joint meniscus and cruciate ligamentbefore and after cut off, not fixed leg drive7days postoperatively, after4weeks for a quick knee arthritis model obviously. All rabbits wererandomly divided into ultrasound irradiation group (group A), oralglucosamine sulfate group (group B), ultrasonic percutaneous penetrationglucosamine sulfate (group C), ultrasonic irradiation plus oralglucosamine sulfate group (group D) and the control group (group E).Open the knee after treatment for4weeks for gross observation, articularcartilage for histologic examination, testing content of hyaluronic acid injoint fluid.RESULTS1. The sound intensity is0.2W/cm2of ultrasound irradiationsupplementary glucosamine sulfate transdermal through and throughrate is the largest. Ultrasonic irradiation not detected in5min groupaccept liquid glucosamine sulfate;2. Histological examination of articular cartilage mankin score, A〠B two groups were significantly higher than group C (P <0.01); GroupA is significantly higher than that of group B is also (P <0.01).3. Group B of the concentration of hyaluronic acid was significant higherthan group A (P <0.01).CONCLUSIONS1. Glucosamine sulfate could not through the skin,1.0MHz ultrasoundirradiation for more than5min, glucosamine sulfate can through rabbitskin, through and through rate increases with the increase of time,through and through rate all increase with the increase of dopeconcentration, different parts of the skin thickness changes do notsignificantly affect the ultrasonic auxiliary effect of glucosaminesulfate solution.2. glucosamine sulfate ultrasonic penetration therapy to inhibit articularcartilage degeneration of the effect is better than that of ultrasonicirradiation.3. Ultrasonic percutaneous penetration glucosamine sulfate can be used asa treatment for osteoarthritis of articular cartilage degeneration of theeffective methods. |
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