| objective:This experiment through somatic cell level andestablish the self-contrast model of rabbit deep second-degreescald,Research the chitosan film spray joint cell growth factor of spray(EGF) on rabbit deep second-degree scald wound healing and the relatedeffect mechanism of wound healing.By observing the wound healing inmacroscopic view,wound healing rate calculation,under the scab bacteriacount,the wound pathological observation,changes in wound tissuehydroxyproline (Hyp) content,ki67in the distribution and the expressionlevel of the wound,to explore the advantage of synergy about treatmentcombination in scald early to middle and its applicationprospect.Methods:Choose healthy New Zealand white rabbit only24,Inrabbit spinal median line,on the back on both sides,use water hot methodto establish six symmetrical rabbit deep second-degree scald,the top twowounds as experimental group(group A:chitosan film former jointEGF),middle two wounds for control group1(group B:EGF),the bottomtwo wounds as control group2(chitosan film former).Group A afterinjury， at first spraying the EGF on the wound,until solution notoutflow,after one minute spraying the chitosan film former on the wound,after being its natural film,bind up and fixed.Group B afterinjury,Only spraying EGF on the wound,until solution not outflow,bindup and fixed.Group C after injury，only spraying chitosan film former onthe wound,after being its natural film,bind up and fixed.Every24hoursreplace the drug and outer dressing,macroscopic observation three groupsin morphological changes and healing of wounds.Selecting observationpoint of3d,7d,10d,14d,each observation point randomly extract sixrabbits,inject the air into the rabbits to death,use weighing paper recordthe wound area,and calculate the rate of wound healing.under the strictaseptic operation,cut out the wound eschar and under eschar tissue mass1.0gram,Processing after bacterial culture,to sum up wound tissuecolonies of bacteria per gram.Then take the100mg rabbit back woundtissue,determination of the wound tissue hydroxyproline (Hyp)content.The rest of the specimen tissue,one parts of the tissue slice andmake HE dyeing,observing pathological indicators,other parts of tissuemake ki67specific antibody dyeing,observe the immunohistochemicalindex.Results:Observing the wound healing in macroscopic view,woundgroup A compared with B and C groups,around the wound inflammationreaction lighter,eschar soft and looser,Wound area reducefaster.Comparison of the wound healing rate,Group A is significantlyhigher than B and C groups, groups B and group C are not obviousdifference between two groups.Histopathological specimens of the wound observe Under the microscope,wound group A compared with B and Cgroups,necrotic tissue attached loose of the wound,inflammatory cellchemotaxis gathered speed,endothelial cells hyperplasiaobviously,collagen fibers hyperplasia active,new epithelium arrangementneatly.Colony Forming Units/gram(CFU/g)positive detection rate ingroup B was higher than group A,Positive rate in group B was higher thangroup C,A and B groups no significant difference.Detection of the woundtissue hydroxyproline (Hyp) content,A group of Hyp content is growingfaster than group B,C group of Hyp content is growing faster than groupB,A and C groups no significant difference.Immunohistochemicalanalysis for ki67,wound group A compared with B and C groups,largenumber of positive cells gathered very closely,the nucleus deepdyeing,arrangement neatly.Compared with the index of ki67positiverate,A and B groups no significant difference,both of the group A andB,compared with the group C,the difference areobviously.Conclusion:Two kinds of drugs used in combination for rabbitdeep second-degree scald wound can be strengthen the ability of woundhealing,and can make up for the defect of single drug.Can fullystrengthen the ability to inhibit bacterial growth,speed up the growth ofepithelial cell proliferation,promote the role of collagen synthesis. |
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