Up-regulation Of SIRT1in Brain Tissue Of Patients And Rats With Temporal Lobe Epilepsy

Objective: SIRT1is NAD+dependent deacetylases and participate in awide variety of importantly physiological and pathological processes.Recently, the effects of neuroprotective and synaptic plasticity of SIRT1have been reported. Here, we analyze the SIRT1expression and deacetylaseactivity level in the brain tissue of epileptic rats and patients with refractoryepilepsy to explore the possible mechanisms of epilepsy.Methods: In the temporal lobe brain tissue of9patients who had headtrauma,15patients with intractable epilepsy, normal control rats and the ratsmodel of TLE after kindling at6h,24h,72h, and on days7,14,30,60,SIRT1expression were detected using immunohistochemistry and westernblotting and SIRT1activity were evaluated using SIRT1Deacetylase AssayKit.Results: In the results of immunohistochemistry, SIRT1predominantly expressed in the cytoplasm of neurons of human and ratsand the strong immunoreactivity of SIRT1was observed in the epilepticgroups compared with the control groups. In the results of western blotting and activity Assay, the expression and activity of SIRT1was significantlyraised in the patients with intractable epilepsy relative to the patients withhead trauma (P<0.05). In the rats model of epilepsy, SIRT1expression wasup-regulated at6h,24h,72h, and on days7,14,30,60after kindling(P<0.05) and SIRT1activity was significantly increased at6h,24h,72h,and on days7,14(P<0.05). The highest expression and activity of SIRT1occurred at72h.Conclusion: Up-regulation of SIRT1expression and activity in thetemporal lobe tissue of patients and rats with epilepsy may play an importantrole in the pathogenesis of epilepsy.