Study On The Induced Differentiation Of Induced Pluripotent Stem Cells Into Cochlear Hair Cell-like Cells And Spiral Ganglion Neuron-like Cells In Vitro

Objective:The mammalian inner ear has limited regenerative ability. The regeneration ofauditory hair cells and auditory spiral ganglion neurons is a principal requirement forpotential cell replacement therapy. In this study, we investigated the potential ofmouse induced pluripotent stem cells （iPSCs）for use as a source of transplants forthe restoration of auditory hair cells and spiral ganglion neurons.Methods:We co-cultured the mouse iPSCs with the cells of the cochlear organ of Corti orthe modiolus in vitro. The cochlear organ of Corti (which contains cochlear hair cells)and the modiolus (which contains auditory spiral ganglion neurons) were obtainedfrom postnatal day3(P3) CD-1ICR mice. After18days of coculture with the cellsof newborn mouse cochleae. The expressions of hair cell markers (MyosinVIIA，Math1， Calretinin， Espin) and Spiral ganglion neuron markers (Nestin、Neurofilament-M、β-Ⅲ Tubulin，Vesicular glutamate transporter1(VGluT1)) weredetected by immunocytochemical analyes.Results:Immunocytochemical analyes resulta indicated that the differentiated iPSCs canexpressed auditory hair cell markers (MyosinVIIA，Math1，Calretinin，Espin)andspiral ganglion markers(Nestin、Neurofilament-M、β-Ⅲ Tubulin， VGluT1).Conclusions:Mouse iPSCs cultured in virto could be successfully induced to differentiate intohair cell-like cells and spiral ganglion-like cells with hair cell and spiral ganglionmolecular markers.