Transforming Growth Facter Beta1Gene Polymorphism And Chronic Obstructive Pulmonary Disease

ObjectiveChronic obstructive pulmonary disease (COPD) was sexual development,quality of life of severely affected patients. With the increasing of atmosphericpollution and the population aging and the smoking population, the prevalenceand mortality of COPD showed an upward trend year by year.Detect therelations on individual susceptibility between the polymorphisms in gene oftransforming growth factor-beta1(TGF-β1) and COPD in Han population.Anal-ysis of TGF-β1gene polymorphism and the severity of airflow limitation inCOPD patients in Han population. To provide a new direction for the clinicaltreatment, so as to effectively reduce COPD morbidity and mortality, reduce thesocial burden.MethodsThe case group was98cases in Qingdao Municipal Hospital RespiratoryDepartment of internal medicine treatment of COPD patients, control group inhealth examination center examination of90cases, genomic DNA extraction oftwo groups of subjects, application of polymerase chain reaction (PCR)-DNAsequencing assay the polymorphisms of+869T/C locus in first exon of TGF-β1gene, analysis of the distribution situation of two groups of genotype and allele，detect the relations on individual susceptibility between TGF-β1gene+869T/Cpolymorphism and COPD in Han population.According to the results ofpulmonary function test in FEV1percentage of predicted value of (FEV1%predicted), determine the flow of patients with COPD limited extent, in patientswith COPD on the basis of airflow limitation grouping, analysis of the TGF-β1gene polymorphism and the severity of airflow limitation in COPD patients in Han population.Results1. In the two groups, the distribution of C allele and T allele werestatistically significant differences (χ2=8.964, P<0.05). In the COPD group, thedistribution of3genotypes CC15.3%, CT58.2%and TT26.5%, in the controlgroup, CC4.4%, CT50%and TT45.6%, were compared between the two groups,the results have significant difference (P<0.05). Logistic regression analysis,CC genotype compared to TT genotype, significantly increased risk of COPD,odds ratio (OR)=5.913,95%confidence interval (CI) for1.916-18.245, P<0.05.2. In COPD patients, between the distribution of TGF-β1gene+869T/Cgenotype and allele frequency in airflow limitation in the packet wasstatistically obvious difference (P<0.05). TGF-β1gene+869T/C site C allelein the severe airflow limitation was higher than that in mild to moderate airflowlimitation (P=0.024). In mild to moderate airflow limitation group, genotypefrequency was CC6.5%、CT58.7%、TT34.8%, in the severe group, genotypefrequency was CC23.1%、 CT57.7%、 TT19.2%, the distribution of CC in thesevere group was higher than that in mild to moderate airflow limitationgroup(P=0.037). CC pulmonary function in patients with FEV1%wassignificantly lower than the expected value of TT type (P<0.05).Conclusions1. TGF-β1gene+869T/C polymorphism may be associated with COPD inHan population, genotype CC is susceptible genotypes of COPD in Hanpopulation.2. TGF-β1gene+869T/C polymorphism and the degree of airflowlimitation may be correlated with COPD in Han population, CC airflowlimitation degree is more serious.