| Purpose:The formation of cerebral infarction, mainly due to the interruption of blood supplyof brain tissue, led to the ischemia hypoxia, produced a series of cascade reaction,eventually led to brain ischemic necrosis, but as a result of thrombolysis treatment, thespontaneous reperfusion and the small vessels of the collateral circulation to re-establish,cerebral ischemia-reperfusion mechanisms are also involved. This study is to show theestablishment of cerebral ischemia-reperfusion rats model, imitating ischemic cascadereaction and ischemic reperfusion injury, which resulted in the process of cerebralinfarction at last.To analyse the clinical application value of ulinastatin on cerebralinfarction, we observe medication time and Ulinastatin in treatment of Infarct volumeafter cerebral ischemia-reperfusion.Method:A total of28healthy male SD rats with MCAO model, were reperfused after130minutes in the middle cerebral artery occlusion.All were randomly divided into four groups，（1）The control group，seven rats，after model establishment, were given2ml saline intraperitoneal injection after3hours.(2)3hours treatment group，seven rats， after model establishment, were givenintraperitoneal injection of ulinastatin after3hours。（Per kilogram of rats to twohundred thousand units of ulinastatin）(3)12hours treatment group，seven rats，aftermodel establishment, were given intraperitoneal injection of ulinastatin after12hours。（Per kilogram of rats to two hundred thousand units of ulinastatin）(4)24hourstreatment group，seven rats，after model establishment, were given intraperitonealinjection of ulinastatin after24hours。（Per kilogram of rats to two hundred thousandunits of ulinastatin）After rats wake up, we score the neurological function of rats withZeal Longa54-point scale.We request neurological score reaching3points.The rats performance the contralateral dump and trouble walking.After all rat MCAO model，dieafter72hours，remove brain tissue。We make2mm slices of brain tissue，then put theminto the TTC to stain。Normal brain tissue appears red color, but the infarction area ofbrain tissue no color.Fixing a digital camera,then taking pictures.Photos will beinputted the Image Pro Plus6.0Image analysis software system, to process calculationof rat infarction volume percent。Its computation formula is: The contralateral normalbrain tissue area minus disease side area of normal brain tissue equal to infarction area，each section multiplies by the sum of the cerebral infarction area thickness (2mm) asthe total volume of infarction.Finally, the percentage of infarct volume is the ratio of theinfarct brain volume of the whole brain infarct volume[5].Analyzing data with SPSS16.0statistical software， applying t test，all data written in （x±s）， P <0.05wasregarded as statistical significant.Results：Compared the treatment group with control group after cerebral ischemiareperfusion，cerebral infarction volume in each treatment group was reduced to varyingdegrees.The cerebral infarction volume of the medication group after3hours wasreduced obviously.3hours and12hours treatment group are compared with controlgroup, treatment group with statistical significance (P <0.05), while the24hourstreatment group is compared with control group,24hours treatment group is nostatistical significance.Conclusions:The ischemia-reperfusion rats within12hours applying ulinastatin can reduceinfarct volume.This time may be effective treatment, time window for ulinastatin animalexperiment. With the continuous progress of the ulinastatin, it may play a significantrole in the clinical treatment. |
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