The Effect Of Berberine Hydrochloride And Atorvastatin Calcium On The Peripheral Blood Basophil Proportion And Basophil Count In Patients With Acute Cerebral Infarction

Objective: We observed the dynamic changes on the peripheral blood basophilproportion and basophil count in patients with acute cerebral infarction (ACI),comparedthe effect of berberine hydrochloride and atorvastatin calcium on the peripheral bloodbasophil proportion and basophil count，discussed the relationship with the degree ofneurologic deficits and the serum level of inducible nitric oxide synthase (iNOS) tofurther explore the effect and its possible pathophysiological mechanism of berberinehydrochloride and atorvastatin calcium in ACI.Method: Healthy check-up of75cases were selected for control group and119patients with ACI were selected for cerebral infarction (CI) group, the patients of CIgroup satisfied the inclusion criteria and exclusion criteria, and two groups werecomparable in age, gender, diabetes mellitus composition. According to the differenttreatments,the CI group was further randomly divided into the standard group (51cases),the berberine group (32cases) and the statin group (36cases) by the control study. Thethree groups were comparable in age, gender composition and the severity of disease.We measured the peripheral blood basophil proportion and basophil count by theJapanese Sysmex XE automated haematology analyser and the compatible reagent. Theserum level of iNOS was measured with the method of chemical colorimetric. Weobserved the change of the peripheral blood basophil proportion and basophil count(×109/L) respectively within48hours of onset (before the treatment) and on the first10days after admission (after the treatment).The loss of neurological function of cerebralinfarction patients was measured by the national institutes of health stroke scale (NIHSS)before and after the treatment. The first data (before the treatment) was marked as “1”,while the second date (after the treatment) was marked as “2”. All date wasanalyzed by SPSS20.0statistics software. The significance level for bilateral test wasless than0.05(P＜0.05).Results:1.The peripheral blood “basophil proportion”1（0.200±0.288）,“basophilproportion”2（0.300±0.300）and “basophil count”1（0.012±0.022）of the CI group hadno significant differences compared with the control group （0.281±0.206，0.017±0.013）.The peripheral blood “basophil count”2（0.020±0.021）of the CI groupwas significantly higher than the control group (P <0.05).2.In the CI group, the peripheral blood “basophil proportion”1,“basophilproportion”2，basophil count “1and “basophil count”2had positive correlation trend(the correlation coefficient is positive)with the twice NIHSS scores (4.070±2.462,2.580±2.338),but had no significant correlation.In the CI group, the peripheral blood “basophil proportion”1and the twice basophilcount had positive correlation trend (the correlation coefficient is positive)with iNOS1（18.007±5.889）、iNOS2（14.411±7.556）, but had no significant correlation. Theperipheral blood “basophil proportion”2had a significant positive correlation withiNOS1、iNOS2（r=0.300,0.251，both P<0.05）.3.Before the treatment, the peripheral blood “basophil proportion”1(0.200±0.250,0.268±0.254,0.252±0.258),the “basophil count”1(0.011±0.017,0.018±0.018,0.015±0.015) and the NIHSS （4.160±2.493,4.340±3.012,3.690±1.818） had nosignificant difference among the standard group,the berberine group and the statingroup.In the standard group, the peripheral blood “basophil proportion”2(0.300±0.350)and “basophil count”2(0.020±0.026) were significant increased than “basophilproportion”1and “basophil count”1（P<0.01）.In the statin group, the peripheralblood “basophil proportion”2(0.393±0.220) and “basophil count”2(0.025±0.013)were significant increased than “basophil proportion”1and“basophilcount”1（P<0.01）.In the berberine group,the peripheral blood“basophilproportion”2(0.285±0.315) and “basophil count”2(0.019±0.021) were slightlyincreased thanbasophil proportion”1and “basophil count”1.The NIHSS2of the standard group, the berberine group and the statin group(3.000±2.698，2.030±2.197，2.530±1.722)were significant decreased than NIHSS1(bothP<0.01). Conclusion:1.The peripheral blood basophil count in patients with ACI increased significantly.It is indicated that the basophil may participate in the pathophysiologic process of ACI.2.The peripheral blood basophil proportion have a significant positive correlationwith the serum of iNOS, indicate that both may have closely correlation in the processof the pathophysiology of cerebral infarction.3. The berberine hydrochloride can inhibit the peripheral blood basophil proportionand basophil count in patients with ACI while the atorvastatin calcium can’t,the exactclinical significance needs further study.