The Anti-tumor Effects Of Thymoquinone On Osteosarcoma SaOS-2Cell Line In Vitro

Objective:To investigate the effect and the mechanism of thymoquinone in the growth inhibition of human osteosarcoma cell line SaOS-2in vitro.Methods:l.The human osteosarcoma SaOS-2cells were treated with different concentration (20,40and80μmol/L) of thymoquinone for24hours, the cellular proliferation was detected by Cell Counting Kit-8(CCK-8) assay.2. After SaOS-2cells were treated with different concentration (20,40and80μmol/L) of thymoquinone for24hours, the flow cytometry (FCM) was used to determine apoptosis in SaOS-2cells.3. The human osteosarcoma SaOS-2cells were treated with thymoquinone at a concentration of80μmol/L for24hours, the morphological changes of SaOS-2cells were observed under the fluorescence microscopy after DAPI staining.4. After SaOS-2cells were treated with different concentration (20,40and80μmol/L) of thymoquinone for24hours, Western blot was used to detect the protein expression of Caspase-3, XIAP and Smac.Results:1. Thymoquinone inhibited the proliferation of SaOS-2cells in a dose-dependent manner, and the proliferation rate was (75.5±4.2)%、(62.1±6.7)%and (52.5Q4.9)%, respectively.2. Thymoquinone induced apoptosis of SaOS-2cells in a dose-dependent manner, and the apoptosis rate was (8.1±0.7)%,(13.2Q1.1)%and (20.2Q1.7)%respectively.3. Thymoquinone induced typical features of apoptosis in SaOS-2cells4. After treatment of thymoquinone, the expression of Caspase-3and Smac were up-regulated in SaOS-2cells, but thymoquinone treatment significantly decreased the XIAP levels in SaOS-2cells.Conclusions:Thymoquinone has the anti-tumor effect to the human osteosarcoma SaOS-2cells in vitro probably by up-regulating the expression of Caspase-3and Smac and down-regulating the expression of XIAP. Five pictures, Two forms, Fifty-four References.