Preliminary Study On The Adenosine Signaling In Bladder Fibrosis

Objective To investigate the mechanism of adenosine signaling in the development of bladder fibrosis through the rat model.Methods Twenty four wistar rats, approximately150grams, were randomly divided into control group, sham operation group, operation group and theophylline group. The sham operation group did midline incision and dissected the bladder neck region without tied down it. The hypodermic injection of theophylline was performed in the theophylline group with10mg/kg/d after the model was established. Then the same treatment continued6weeks when the rats were killed. Immunohistochemistry and real-time PCR were used to detect the expression of TGF-β1,5-ectonucleotidase(CD73), types I(Col Ⅰ) and Ⅲ (Col Ⅲ) collagens.Results Compared with the sham group, the expression of TGF-β1,5-ectonucleotidase(CD73), types I(Col Ⅰ) and Ⅲ (Col Ⅲ) collagens in control group were not statistically significant(P>0.05).Compared with the control group, the expression of TGF-β1,5-ectonucleotidase(CD73), types I(Col Ⅰ) and Ⅲ (Col Ⅲ) collagens in operation group was statistically significant (P<0.05). Compared with the operation group, the expression of TGF-β1,5-ectonucleotidase(CD73), types I(Col Ⅰ) and Ⅲ (Col Ⅲ) collagens in theophylline group was statistically significant (P<0.05).Conclusions Firstly, the model of bladder outlet obstruction induced detrusor fibrosis of bladder. Secondly, theophylline,the adenosine receptor antagonists, inhibited the progress of detrusor fibrosis of bladder. Finally, the inhibitor of5-ectonucleotidase(CD73)mRNA maybe feedback by the expression of adenosine.twenty-one figures,five tables,forty-nine references.