Detecting The Expression Of Multiple Signaling Pathways In Prostate Cancer

Objective:To understand the relationship between prostate cancer-related genes, analyze the interactive network of biological molecular in prostate cancer from a system perspective, reveal the dynamic process in the development of benign prostatic hyperplasia to prostate cancer and provide new clues for the screening of therapy programs in prostate cancer, through investigating the expression of the key genes in prostate cancer-associated signaling pathways.Methods:The key genes of prostate cancer-related signal pathways were screened by the gene chip from the Qiagen Company of Germany.At the same time,eight cancer associated genes’mRNA in benign prostatic hyperplasia and prostate cancer were detected through FQ-PCR (Fluorescent Quantitative Polymerase Chain Reaction). The data were statistically analyzed with the SPSS15.0software.Results:Seventeen interest genes were screened by RT2Profiler PCR Array,then input to the online gene network database.To our surprise,there was a mimic diagram of gene network,which consisted of eight prostate cancer-associated genes. AKT1and EGFR was the central node of the interactive network.The relative expression level of eight cancer-related genes was statistically significant (P<0.05) in benign prostatic hyperplasia tissue and prostate cancer tissue.Conclusions:Seventeen genes of interest,such as EGFR,FASN and GSTPl,may be closely related to development and metastasis in prostate cancer. A mimic diagram of gene network consisted of eight prostate cancer-associated genes,such as AKT-1,KLK-3and IGF-1. The crosstalk among the target genes made the pathogenesis in prostate cancer became more complex.As a central node of the genic network control, AKT1and EGFR can be used as a preferred target in gene therapy for prostate cancer.