| ObjectiveTo investigate the effect of irbesartan pretreatment on myocardialischemia/re-perfusion injury in rats and its possible mechanismMethodsSelect40healthy male SD rats with3-4months, and adapt to feed oneweek to randomly divided into three groups: shame group (group A) eight rats,ischemia/re-perfusion group (group B)16rats, and irbesartan pretreatmentgroup (group C)16rats; group A and group B rats were gavaged normalsaline5ml/kg everyday, group C rats were garaged irbesartan with30mg/kgmatching normal saline for1week. After they were anesthesiaed and fixed onoperation table, ligation in the anterior descending coronary artery for45minand loosen for120min to establish rat myocardial ischemia/re-perfusion (I/R)model. To observe and measure myocardial infarction area with TTC staining,To observative myocardial cell structure and inflammatory cells infiltration withHE dyeing, after re-perfusion we take blood serum separation by colorimetricmethod to detect serum malondialdehyde (MDA) concentration, biochemicaldetection of myocardial cell necrosis markers TNI content, in ELISA method todetect serum tumor necrosis factor (TNF-α) and interleukin6(IL-6)concentration.ResultsFrom the pathological morphology observation, aggravate myocardial injury after reperfusion, irbesartan pretreatment can reduce myocardialcell edema, improve the myocardial cell structure, reduce inflammation cellinfiltration. In the serum IL-6, TNF-α, MDA, cTnI, control group were comparedwith reperfusion group P<0.01, the control group compared with irbesartanpretreatment group P<0.01, the reperfusion group compared with irbesartanpretreatment group P<0.01. Myocardial infarction area of irbesartanpretreatment group significantly reduced compared with ischemia reperfusiongroup (P<0.01).ConclusionsIrbesartan pretreatment has certain protective effect to rat myocardialischemia/re-perfusion injury, That may be by reducing lipid peroxide, stablingcell membranes, and reducing inflammation factor synthesis or releasing. |
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