Investigation On Extraction And Purification Process Of Cucurbitacin B From Cucumis Melo L

ObjectiveTo study the extraction and purification process of Cucurbitacin B fromCucumis melo L,determine the content of Cucurbitacin B in the best condition ofextraction and purification technology, preliminary discuss the preparative liquidchromatography purified Cucurbitacin B.MethodsThe reflux extraction,ultrasonic extraction and the supercritical CO₂extraction were taken to study the best extraction conditions of Cucumis meloL,measuring the extraction rate of Cucurbitacin B by high performance liquidchromatography,by uniform design experiment preferred optimum extractionprocess and use statistical methods for analysis of experimental results.Use themacroporous adsorptive resin by the purification Cucurbitacin B and confirm thebest purification process.Preparative high performance liquid chromatographywas used in the further purpification experiment to get higher purity.ResultsWith the supercritical CO₂extraction,the Cucurbitacin B content reaches16.46%,higher than the method of reflux extraction11.59%and the method ofultrasonic extraction13.52%.Optimal extraction technology was as follows:extracting pressure of26.0MPa,extracting temperature of55℃,dynamicextracting for3.5h.The purity of Cucurbitacin B could reach79.93%in thecondition of1mL·min-1speed sampling,70%ethanol as eluent,1mL·min-1speedfor elution.Preparative high performance liquidchromatography was used in the further purpification experiment,the purity of Cucurbitacin B could reach above95%.ConclusionsThe result of supercritical CO₂extraction in the best condition was betterthan the result of reflux extraction and ultrasonic extraction, it is a new avenuefor the study of Cucumis melo L.Though the purification of the macroporousresin the content of the Cucurbitacin B is elevated. Preparative highperformance liquid chromatography was used in the further purpificationexperiment and get higher purity.