The Study On Expression And Intervention Of MCP-1in Kidney Of Diabetic Nephropathy Rats

Objective:1. To establish the type2diabetic nephropathy rats model,exploring the correlationbetween the expression of the rat’s kidney MCP-1and Diabetic nephropathy.2. Observe the influence of proteinuria and Renal pathological changes afterirbesartan and probucol have the intervention on type2diabetic nephropathy rat’skidney tissues express MCP-1.Method:Take606-week-old clean grade male SD rats to divide randomly into twogroups: the NC control group (n=10)to be ordinary diet,model group(n=50)to givehigh-sugar,high-fat and high-cholesterol diet. After8weeks,the model rats wereinjected Streptozotocin（STZ）40mg kg-1into intraperitoneal. After72hours,detectingweight,random blood glucose,24-hour urine volume and24-hour urineprotein(24hUTP). The38SD rats were divided randomly into diabetic nephropathy（DN=13）,irbesartan group (DI=13),irbesartan and probucol group (DP=12);DIgroup with irbesartan(150mg kg-1d-1) gavage,DP group with probucol (500mg kg-1d-1) and irbesartan(150mg kg-1d-1) gavage. After8weeks of monitoring andrecording every group in weight,random blood glucose,blood lipids,renalfunction,24-hour urine volume and24-hour urine protein.Use light morphology toobserve general structure of kidney and electron microscope. Observe superfine renalultrastructure;monitoring MCP-1protein expression with immunohistochemicalmethod and detecting MCP-1mRNA expression of the kidney with RT-PCR method.Result:1. Compared with the NC group,DN group rats increased feeding,lack of energy, slowactivity, increased urine output,loss of weight,body hair messy dull. after theintervention two groups situation more than improved.2.Compared with NC group,all of groups the rats of24h urine volume,24hUTP, random blood glucose,blood muscle anhydride (Scr), urea nitrogen (BUN),serumcholesterol (TC)and triglycerides （TG） were increased significantly,loss ofweight(P<0.05);Compared with DN group,DI group the rats of random bloodglucose,BUN,Scr,TG,TC has no significant changed,DP group the rats of randomblood glucose,BUN,Scr has no significant changed(P>0.05),DI group and DP groupput on weight (P<0.05),The rest of the indicators were obviously decreased(P<0.05);Compared with the DI group,DP group the rats of random bloodglucose,BUN,Scr,weight has no significant changed(P>0.05),The rest of theindicators were obviously decreased(P<0.05).3.Compared with NC group,all of groups the rats Kidney tissue pathology wereexacerbated,expression of MCP-1mRNA and protein in the renal tissues wereincreased significantly (P<0.05);Compared with DN group,DI group and DP groupKidney tissue pathology were improved,expression of MCP-1mRNAand protein inthe renal tissues were decreased (P<0.05);Compared with DI group, DP group Kidneytissue pathology were improved,expression of MCP-1mRNA and protein in the renaltissues were decreased (P<0.05).Conclusion:1.The MCP-1excessive expression of diabetic nephropathy renal tissue,MCP-1playan important role in the occurrence and development of diabetic nephropathy.2. after irbesartan treatment can obviously decrease diabetic nephropathy rats kidneyexpress MCP-1,To improve theurine protein and kidney pathological damage in thediabetic nephropathy rat,It has certain effect of protective in diabetic kidneydisease,after the intervention with probucol,it has synergy on the protection of thediabetic nephropathy.