Investigation On The Secreted Frizzled-related Protein5Levels And Their Relationships With Insulin Resistance Related Parameters In Obesity And Type2Diabetes Mellitus Patients

Objective: To detect plasma secreted frizzled-related protein(SFRP)5levels in healthy, obese and newly diagnosed type2diabetesmellitus(T2DM) subjects and to investigate the relationships betweenplasma SFRP5levels and body fat parameters, insulin resistance(IR)，glucolipid metabolism and inflammation.Method: Eighty-nine subjects with normal glucose tolerance (NGTgroup) and eighty-seven patients with T2DM (T2DM group) were enrolledand each group was divided into normal weight (NW) and obese (OB)subgroups. Obesity was defined as BMI≥25kg/m2according to theWHO-Western Pacific Region diagnostic criteria (2000). Body fatparameters were measured and BMI, waist-to-hip were evaluated.Meanwhile the levels of blood glucolipid parameters and fasting insulin(FINS) were also detected. IR index was assessed by homeostasis modelassessment (HOMA). The levels of plasma SFRP5and interleukin (IL)-6 were detected by ELISA.Result:①P lasma SFRP5levels were lower in T2DM group than inNGT group (p＜0.01), the levels of plasma SFRP5in subjects withobesity were also lower than those in subjects with NW in both NGT andT2DM groups(p＜0.01), T2DM-OB subgroup had lower plasma SFRP5levels than that in NGT-OB subgroup (p＜0.01);②Correlation analysisshowed that plasma SFRP5levels were negatively correlated with WHR,HbA1C, FINS, triglycerides, waist circumference, fasting plasma glucose,IL-6, HOMA-IR and BMI(p＜0.05or p＜0.01), Multiple linear regressionanalysis showed that HOMA-IR, BMI, triglycerides were independentlyand negatively related with plasma SFRP5levels(p＜0.05);③Logisticregression analysis showed that SFRP5levels were negatively correlatedwith IR and T2DM, OR＜1（p＜0.05or p＜0.01）.Conclusion: Plasma SFRP5levels were decreased in obese andT2DM subjects and were correlated with body fat parameters, glucolipidmetabolism, IR and inflammation; IR, BMI and triglycerides wereindependently factors that affected SFRP5levels; SFRP5was a protectivefactor that improved IR and prevented T2DM, it may contribute to thepathophysiology of obesity and T2DM.