Oxidative Low Density Lipoprotein Increases OX40L In Endothelial Cells Through A Lectin-like Oxidized Low-density Lipoprotein-1Dependent Mechanism

Objective: To investigate whether oxidative low density lipoprotein(ox-LDL) regulates OX40L expression through a lectin-like oxidizedlow-density lipoprotein-1(LOX-1) mediated mechanism.Methods: Detect the effect of different concentration of ox-LDL onproliferation of cultured HUVEC by CCK-8to filter the suitableconcentration of ox-LDL-induced HUVECs injury; Detect cell cycle andapoptosis rate Using flow cytometry; Experimental groups：ox-LDL group,ox-LDL+Poly I group and normal control group, detect OX40L proteinexpression by Western blot and immunofluorescence and detect OX40LmRNA expression by real-time quantitative PCR (RT-qPCR).Results: As the concentration of ox-LDL increases, proliferation ofHUVEC reduced and we elect100ug/ml as the experimentalconcentration.Compared with the control group, Cell of100ug/ml ox-LDLproup block in S stage and apoptosis rate increase. Compared with thecontrol group, OX40L protein and mRNA expression of ox-LDL group increase. Compared with the ox-LDL group, OX40L protein and mRNAexpression of ox-LDL+Poly I group decrease. OX40L express in cellmembrane of HUVEC.Conclusions: There is a clear role of ox-LDL on damage of HUVEC.Ox-LDL can promote the protein and mRNA expression of OX40L onHUVECs and LOX-1may play a mediating role, affecting OX40/OX40Linflammatory signaling pathways.