Study On The Action Mechanism Of Zhengshi Sanhao’jiegu Powder In The Process Of Rabbit’ Fracture Healing

Objective:TO study treatment effect and possible action mechanism ofZhengshi Sanhaojiegu Powde on the healing of fracture.Methods：30New ZealandRabbits were randomly divided into two groups two weeks later after the success of themodel operation:Blank comparison group(A), Zhengshiyihaojiegusan group(B)， theneach group was divided into3groups according to the7th、14th、21st after theadministration，5rats each group. The rats of A group was administered with the yarn(W4cm, long6cm,0.2cm thick) parceling fractures per day. B group was administeredwith spreaded "Zhengshi Sanhaojiegu Powder " party yarn (width4cm, long6cm,0.2cm thick)parcelling fractures per day. To collect heart blood of5New Zealandrabbits each group After7th、14th、21st day of the administration (i.e.21th,28th,35th day after fracture), and take air embolism method executed after the radialperturbation, X ray to micro electronic balance weighing radial weight, callus weight.Based on callus growth and trace elements in serum calcium (Ca), phosphorus (P),copper (Cu) content, to explore the effect of Zhengshi Sanhaojiegu Powder on thecallus and Ca, P,Cu in the serum in the process of Rabbit’ Fracture Healing. Results：①The growth of rabbit body weight changes: animals fed into the laboratory after theadaptation period, the weight remained fairly stable; modeling, the apparent weight ofexperimental animals downward trend; week after modeling showed steady increase inbody weight after the start of the state.②X-ray observation: Time points after modeling,experimental drug group callus formation were better than the control group, especiallyin the administration of2weeks (28th day after the fracture) was observed after themost obvious when, X-ray showed a clear calcium-based bone marrow cavity filledwith a lot of callus and closed fracture line blurred. Administration of3weeks (ie35thday after fracture) after the callus into the shaping period, but the control group of callusis still in the mature stage of reconstruction.③the weight of callus weighing results:Each time point, the test drug group average of the results are of callus weighing morethan the control group, and the difference in the two weeks after administration of the most significant.④New Zealand white rabbits of experimental fracture trace elementsin serum Ca content of the test results showed that: Experimental drug group in theaverage content of each phase are higher than the control group, and1week after drugadministration and after administration of3weeks (ie21th,35th day after fracture), themeasured average serum levels of Ca significantly higher than the control group.⑤New Zealand white rabbits of experimental fracture trace elements in serum P contentof the test results: experimental drug group in the average content of each phase arehigher than the control group, and2weeks after drug administration (ie,28th day afterfracture) when measured in serum was significantly higher than the average P content inthe control group. Two groups of experimental animals (rabbits) at all time points ofserum phosphorus content of trace elements in the test results mean statistical test P<0.05, significant difference was statistically significant.⑥New Zealand white rabbitsof experimental fracture trace elements in serum Cu content of the test results showedthat: the experimental drug in serum Cu content of trace elements in the average timeincreases with the dose at first and then decreased trend, that is, after administration of2weeks (28th day after fracture) measured serum levels of Cu than the average one weekafter administration (ie,21th day after fracture) and3weeks (35th day after the fracture)is higher. However, Zhengshi Sanhaojiegu Powder group in each phase of the contentwere higher than the control group, the difference was statistically significant (P<0.05).Conclusions：①Zhengshi Sanhaojiegu Powder could speed up callus formation,maturation, alterations and shaping, shorten the healing time, and apparently promotefracture healing in experimental fracture healing process of the New Zealand Rabbit.②Zhengshi Sanhaojiegu Powder Can significantly increase Ca, P, Cu content in the serum,adjust the microelemen’ levels in the serum, and accelerated calcium salt’deposition onthe callus.This may be the mechanism of action of promoting fracture healing.