Effects Of Intrauterine Hypoxia On The Mechanisms Of Learning And Memory In Rats And Ferulic Acid Regulation

Objective To discuss the effects of intrauterine hypoxiaon higher brain function of learning and memory in rats,at the same timeJmjd6and SYN（Synaptophysin）have express the hippocampusneurons in young rats; to discuss Ferulic acid in which the regulatory rolein juvenile rats. Method: Healthy pregnant14thdays of SpragueDawley rats(n=30) were randomly separated into hypoxia group(n=10), Ferulic acid (n=10),control group (n=10) group. The Ferulicacid group and hypoxia group pregnant rats were placed in thehypobaric and hypoxia experimental animals chamber (Oxygenpartial pressure13kpa, tribute pressure488mmHg, a simulatedaltitude of3600m, Temperature:25℃, Relative humidity:75%～80%）starting from pregnant14thdays for2hours a day for3successive days(gestational14-16th days),to establish theintrauterine hypoxia model of fetal rats. Detected hippocampalneurons of new rat and juvenile rat of Jmjd6mRNA and SYN-ⅠmRNA expression by in situ hybridization; and then Detectedlearning and memory abilities of juvenile rats using the Morriswater maze; detect Jmjd6and SYN expression in the hippocampalneurons of juvenile rat by means of Western blot. Results: Thequantity of the integral optical density(IOD) value of hippocampal neuron Jmjd6mRNA expression in hypoxia group new rats andjuvenile rats were extraordinary higher than those of controlgroup(P＜0.05), and also Ferulic acid group was slightly lower thanhypoxia group (P＜0.05).The IOD value of positive SYN-Ⅰ mRNAcells of hippocampal CA3in hypoxia group new rats and juvenilerats were significantly lower than control group, and also Ferulicacid group was extraordinary higher than that of hypoxia group (P＜0.05).In the Morris water maze, the escape latency of hypoxiagroup was prolonged compared with that of control group (P＜0.05), Ferulic acid group was shorter compare with that of hypoxiagroup (P＜0.05); the searching time of the Spatial positioningnavigation of hypoxia group was extraordinary shorter contrast tothe control group(P＜0.05),at the same time, Ferulic acid groupwas lengthen than the hypoxia group(P＜0.05).by means ofWestern blot to be discovered Jmjd6expression in hippocampalneurons of hypoxia group juvenile rats were extraordinary higherthan those of control group(P＜0.05), and also Ferulic acid groupwas slightly lower than hypoxia group (P＜0.05). SYN expression inhippocampal neurons of hypoxia group juvenile rats weresignificantly lower than those of control group(P＜0.05), and alsoFerulic acid group was slightly higher than hypoxia group (P＜0.05).Conclusion: Learning and memory can be caused to be reduce by Intrauterine hypoxia in juvenile rats. The mechanismmay be related to the increasing expression of Jmjd6so that theSYN expression was decrease. Ferulic acid injection could reducethe damage to neurons by hypoxic and improve the learning andmemory in juvenile rats. The mechanism may be associated withreduced expression of hippocampal neurons Jmjd6so that SYNexpression increased.