The Research Of Expression And Clinical Significance Of SOX7、β-catenin And Cyclin D1Protein In Invasive Breast Cancer

Objective:This study aimed to investigate the expression of SOX7, β-catenin and cyclin D1in invasive breast cancer and hyperplastic disease of the breast, and explore their relationship with clinical pathology charactersis in invasive breast cancer in order to provide valuable biomarkers for the treatment and prognosis.Methods:The expression of SOX7, P-catenin and cyclin D1was measured in50invasive breast cancer tissues and30hyperplastic disease of the breast by immunohistochemical SP method. The correlations of SOX7, β-catenin and cyclin D1in invasive breast cancer to clinicopathologic features were analyzed, such as age, size of tumor, axillary lymph node metastasis, histological grade, pTNM stage, ER, PR, Her-2expression and the risk.Results:1. Immunohistochemical results showed that the positive rates of SOX7and cyclin D1in invasive breast cancer were42%(21/50) and70%(35/50), and the abnormal expression rates of P-catenin in invasive breast cancer was70%(35/50). The positive rates of SOX7in invasive breast cancer was significantly lower than that in hyperplastic disease of the breast70%(21/30). The expression of SOX7had difference between two groups (P=0.021<0.05). The abnormal expression rates of P-catenin and the positive rates of cyclin D1in invasive breast cancer were significantly higher than that in hyperplastic disease of the breast43.3%(13/30)(P=0.033<0.05) and20%(6/30)(P=0.000<0.001). The expression of β-catenin and cyclin D1had difference between two groups.2. In invasive breast cancer, SOX7and β-catenin protein expression in12cases,while negative expression in5cases; SOX7and cyclin D1protein positive expression in11cases, while negative expression in5cases; P-catenin and cyclin D1protein positive expression in28cases, while negative expression in8cases. Results of the analysis by Spearman showed that in invasive breast cancer the SOX7protein expression was negatively correlated with the abnormal expression of β-catenin protein and the expression of cyclin D1protein (r=-0.282, P=0.046<0.05; r=-0.327, P=0.020<0.05) while the abnormal expression of P-catenin protein was positively correlated with the expression of cyclin Dl protein (r=0.333, P=0.018<0.05)3. In invasive breast cancer the expression of SOX7protein was correlated with age, axillary lymph node metastasis, histological grade, pTNM stage, ER, PR, Her-2expression and the risk(P<0.05), but with no correlation with size of tumor (P>0.05).However, the abnormal expression of β-catenin and the positive expression of cyclin D1in invasive breast cancer were correlated with size of tumor, axillary lymph node metastasis and pTNM stage(P<0.05), but with no correlation with age, histological grade, ER, PR, Her-2and the risk(P>0.05).Conclusions:1. SOX7, P-catenin and cyclin Dl are frequently abnormality-regulated in invasive breast cancer tissues, indicating their involvement in the development and progression of invasive breast cancer.2. In invasive breast cancer the SOX7protein expression was negatively correlated with the abnormal expression of β-catenin protein and the expression of cyclin D1protein while the abnormal expression of β-catenin protein was positively correlated with the expression of cyclin D1protein. The three protein may play a regulatory role through the same pathway in the development and progression of invasive breast cancer.3. SOX7,β-catenin and cyclin D1may be valuable marker for assessing the prognosis for invasive breast cancer.