The Effect On Expression Of BMI-1and P16^INK4A In Human Epithelial Ovarian Cancer Cell Lines HO-8910Exposed To DDP

Objective:To investigate the effect on expression of BMI-1and P16INK4A in human epithelial ovarian cancer cell lines HO-8910exposed to DDP,then to explore the relationship between BMI-1,P16INK4A and apoptosis of ovarian cancer cells.Methods:1. When HO-8910cells reached the logarithmic growth phase, the cells were exposed to DDP at different concentrations for24h and48h and72h,Then MTT method was used to detect the inhibition rate of cells.2. When HO-8910cells reached the logarithmic growth phase, the cells were exposed to DDP at different concentrations for48h, Then TUNEL assay was used to detect the occurrence of apoptosis in the cells.3. Western blotting were used to detect BMI-1and P16INK4A expression in HO-8910cells at logarithmic growth phase after they were exposed to DDP at the required concentration for48h.4. Real-time PCR were used to test the expression of BMI-1mRNA and P16INK4AmRNA in HO-8910cells after they were exposed to DDP at the required concentration for48h. Results:1. The concentration of DDP ranging from0.3125ug/ml--10ug/ml can inhibit proliferation of HO-8910cells in vitro significantly in time-dose-dependent fashion (P<0.05).2. TUNEL test proved that DDP can induce apoptosis of ovarian cancer cell HO-8910, with the increase of drug concentration the positive signal was enhanced, suggesting that the number of apoptosis cells was increased accordingly.3. After the cisplatin intervention on ovarian cancer cell line HO-8910cells for48h, the relative expression of protein of BMI-1and、 P161NK4A were decreased, compared with no drug group, the difference was statistically significant.(P<0.05).4. After the cisplatin intervention on ovarian cancer cell line HO-8910cells for48h, the relative expression of mRNA of BMI-1and P16INK4A were decreased, compared with the no drug group, the difference was statistically significant.(P<0.05).Conclusion:1. In a certain concentration range, DDP can inhibit ovarian cancer cell line HO-8910cells in time and concentration-dependent manners,It also can induce apoptosis of ovarian cancer cell HO-8910.2. The expression downregulation of BMI-1and P16INK4A in apoptosis of ovarian cancer cell HO-8910induced by DDP, suggesting that the two genes may be involved in the process of apoptosis in ovarian cancer cells, and more researches are needed to verify is regulation mechanism.