Polymorphisms In Three Selenoprotein Genes And Risk Of Hemorrhagic Fever With Renal Syndrome In Chinese Han People

Objective:To find out the relationship between genetic variations of selenoprotein and HFRS susceptibility to show the influence of selenoprotein system in genetics of HFRS.Methods:We studied in Chinese Han people. Individuals were genotyped5SNPs in199HFRS cases and403health people as controls. Methods PCR-RFLP and ARMS PCR were used to assayed these gene variations in a minimum of HFRS cases and controls. Plasma GPx3levels were determined by ELISA. SPSS16.0, Haploview4.2, SHEsis were used to analysis the data.Result:The genotype frequencies of GPxl rs1050450(Pro200Leu) were significantly different between cases and controls (P=0.048). Use multiple conditional logistic regression analysis adjusted for sex, age and immune response, GPx1rs1050450(Pro200Leu) TT genotype was associated with an increased risk for HFRS (OR-2.66,95%CI=1.13-6.24, P=0.025), compared with CC genotype. For GPx1gene, TT haplotype of rs1050450and rs1800668was significantly associated with HFRS susceptibility (OR=1.75,95%CI=1.06-2.86,P=0.027). For GPx3gene, TT (OR=1.59,95%CI=1.01-2.50, P=0.046) and GC (OR=2.08,95%CI=1.03-4.35, P=0.041) haplotype of rs2233311and rs8177412were significantly associated with HFRS susceptibility. The plasma GPx3level was significantly different between the HFRS cases and controls (P<0.001). Compared with the CC genotype, HFRS patients carrying GPx1rs1050450(Pro200Leu) T allele has a lower plasma GPx3level (P=0.038). HFRS patients carrying GPx1rs1800668(-46C/T) T allele has a lower plasma GPx3level compared with the CC genotype (P=0.038).Conclusion:Our study found that Selenoproteins GPx1, GPx3genes polymorphisms are significantly associated with HFRS susceptibility. GPx1genotype was associated with serum GPx3level. The results interpret hereditary factor maybe play an important role in HFRS infection.