The Expression Of TRIP6in The Hippocampal Formation Of A Rat Model For Temporal Lobe Epilepsy

Objectives:To investigate the the expression of TRIP6in the hippocampal formation of a rat model for temporal lobe epilepsy (TLE).Methods:46healthy adult male Sprague-Dawley(SD) rats were used for the study. The rats were divided randomly into normal group(n=6) and experimental group(n=40). The temporal lobe epilepsy model was established by intraperitoneal injection of lithium chloride-pilocarpine, while the normal group were injected with equal dose of saline. After30days, six of the rats developed spontaneous recurrent seizures were randomly enrolled into TEL gruop, another six of the rats without spontaneous recurrent seizures were randomly enrolled into nTEL group. The animals were perfused with4%formaldehyde. The thickness of every sections was40μm, and took one in every four sections for TRIP6detection. After immunofluorescence staining, the expression of TRIP6in hippocampal cell layers(DG, CA3and CA1) and nerve fibers were observed using laser scanning confocal microscope, and the positive fluorescence signal were detected using imageJ analysis software. Results:(1) By immunofluorescence staining, we found TRIP6was mainly expressed in fibers region in normal group; while in TLE and nTLE group, it was expressed in fibers region and cell layer.(2) The positive immunostaining of TRIP6in the cell layer of hippocampal formation was colocalized with DAPI signal which labeled the nucleus.(3) The expression of nuclear TRIP6in the hippocampus(DG, CA3and CA1) was significantly upregulated in TLE and nTLE group (p<0.01), moreover, the expression of nuclear TRIP6in TEL group was markedly more than that of nTLE group (p<0.01). In fibers region, the expression of TRIP6in CA3and CA1region was showed as follows:TLE group>nTLE group>normal group(p<0.01), while expression of TRIP6in DG region:TLE group<nTLE group<normal group(p<0.01).Conclusions:The relatively specific expression feature of TRIP6in hippocampal formatoin is closely related to the epileptogenesis of temporal lobe epilepsy, may be the important molecular of anti-epilepsy formation.