The Neural Mechanisms Of Cross-model Memory Induced By Associative Learning Memory

Objective: By establishing animal model of mice on whisker stimulation witholfactory stimulation associative learning memory for cross mode memory，we aim toanalyze the mechanism of cross model memory and retest the cytological mechanismof cross model memory.After the model of associative learning memory established,we record the barrel cortex and piriform cortex respectively in response to olfactorysignals and whisker tactile signals.Methods:1. The establishment of the animal model of conditioned reflex:Divide intomice beard swing test and T maze test. Experimental animal use15days after thebirth of the Kunming mice, divided into two groups: control group (n=13) andcombined training group (n=16), blank control group was not trained. The training setof training patterns is in butyl acetate as the odor source of olfactory stimulation,mechanical vibration to beard the stimulation of5Hz. Application of Canon HFR28video camera shooting was conducted on mice in the beard swing butyl acetate odorstimulation, use VideoStudio soft analysis frequency change mouse vibrissa swing. Tmaze test was administered to mice in mechanical vibration beard5Hz stimulation,changes in selectivity to T maze arms in mice.2.Local field potential experiment:15days after the birth of the Kunming mice,divided into blank control group (n=13) and combined training group (n=16), blankcontrol group was not training process, training group of continuous10days oftraining. The experimental mice were anesthetized by intraperitoneal injection of20%urethane, urethane anesthesia dosage is0.1ml/10g, should not be too deep, cut openthe mouse head skin, exposed skull, rapid mouse fixed in rat brain stereotaxicapparatus, on mouse barrel cortex using rat brain stereotaxic instrument (BarrelCortex) and the piriform cortex (Piriform Cortex) of brain location, accuratepositioning after craniotomy operation. Using NARISHIGE motorized manipulator will electrodes are inserted into the barrel cortex (Barrel Cortex), collecting fieldpotential signals using Axoclamp-200B amplifier; also the electrode in piriform cortex(Piriform Cortex), recording field potential signals in mice were given5Hzmechanical vibration stimulation and beard odor stimulation conditions.Result:1. Behavioral results: Mice in an olfactory Beard-joint training for10days toestablish with butyl acetate odor stimulation as the conditioned stimulus combinedlearning and memory, manifested in butyl acetate increased as the olfactory stimulusbeard oscillation frequency under the condition of. In the mechanical vibration as thetactile T maze test conditions, the mice in the training group chose to avoid the smellof butyl acetate only number than the normal group, and compared with the normalgroup there were significant differences.2. LFP results:(1)Insert the electrode in a mouse model of barrel cortex, give thebuty acetate odor stimulation, a potential signal appeared; mechanical vibration tobeard5Hz stimulation, there is a potential signal.(2)In the model mice of piriformcortex (Piriform Cortex) is inserted into the electrode, mechanical vibration to beard5Hz stimulation, there is a potential signal to appear; butyl acetate odor stimulation,there is field potential signals.(3)In normal mouse barrel cortex into the electrodeand butyl acetate odor stimulation, no field potential signals of mechanical vibration;give beard5Hz stimulation, there is a potential signal.(4)In normal mouse piriformcortex mechanical vibration into the electrode at the same time give beard5Hzstimulation, no field potential signals to appear; butyl acetate odor stimulation, thereis field potential signals.Conclusion:1. Mice in accepting the beard touch smell joint training for a period oftime, can establish a conditioned reflex formation of combined learning and memory.2. Mice under beard touch smell combined training, forming a combination oflearning and memory, touch and smell the corresponding area of barrel cortex andPiriform Cortex appeared between the cross sensory response, may form a link.3. Weconclude that combined the learning and memory may be better than the simpleexperience dependent learning and memory.